Stimulation of the locus coeruleus elicits noradrenaline and dopamine release in the medial prefrontal and parietal cortex
Stimulation of the locus coeruleus elicits noradrenaline and dopamine release in the medial prefrontal and parietal cortex
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DOI：
10.1111/j.1471-4159.2004.02866.x
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发表时间：
2005-01-01
影响因子：
4.7
通讯作者：
Gessa, GL
中科院分区：
文献类型：
--
作者：
Devoto, P;Flore, G;Gessa, GL
我们以前的研究表明，多巴胺和去甲肾上腺素可能是从大脑皮层的去甲肾上腺素能神经末梢共同释放的。为了进一步阐明这个问题，在自由活动的大鼠中，通过微透析分析了电刺激蓝斑对内侧前额叶皮层、顶叶皮层和尾状核细胞外去甲肾上腺素、多巴胺和DOPAC的影响。以1 Hz的均匀间隔脉冲刺激蓝斑20 min未能改变皮质儿茶酚胺和DOPAC水平。在12和24赫兹的脉冲串刺激增加，在频率相关的方式，不仅去甲肾上腺素，但也多巴胺和DOPAC在两个皮质。在这两个皮质去甲肾上腺素恢复到基线内20分钟的刺激，无论刺激频率，而多巴胺恢复到正常20和60分钟内，内侧前额叶皮层和60和80分钟内顶叶皮层后，12和24赫兹的刺激，分别。DOPAC在整个实验期间保持升高。在12 Hz时相刺激蓝斑增加去甲肾上腺素在尾状核在大脑皮层，但完全无效的多巴胺和DOPAC。河豚毒素灌注到内侧前额叶皮质显着降低去甲肾上腺素和多巴胺水平，抑制电刺激的效果。这些结果表明，电刺激引起的多巴胺增加是一个神经冲动胞吐过程，并建议皮质多巴胺和去甲肾上腺素可能是共同释放的去甲肾上腺素能末梢。
Our previous studies have suggested that dopamine and noradrenaline may be coreleased from noradrenergic nerve terminals in the cerebral cortex. To further clarify this issue, the effect of electrical stimulation of the locus coeruleus on extracellular noradrenaline, dopamine and DOPAC in the medial prefrontal cortex, parietal cortex and caudate nucleus was analysed by microdialysis in freely moving rats. Stimulation of the locus coeruleus for 20 min with evenly spaced pulses at 1 Hz failed to modify cortical catecholamines and DOPAC levels. Stimulation with bursts of pulses at 12 and 24 Hz increased, in a frequency-related manner, not only noradrenaline but also dopamine and DOPAC in the two cortices. In both cortices noradrenaline returned to baseline within 20 min of stimulation, irrespective of the stimulation frequency, whereas dopamine returned to normal within 20 and 60 min in the medial prefrontal cortex and within 60 and 80 min in the parietal cortex after 12 and 24 Hz stimulation, respectively. DOPAC remained elevated throughout the experimental period. Phasic stimulation of the locus coeruleus at 12 Hz increased noradrenaline in the caudate nucleus as in the cerebral cortices but was totally ineffective on dopamine and DOPAC. Tetrodotoxin perfusion into the medial prefrontal cortex dramatically reduced noradrenaline and dopamine levels and suppressed the effect of electrical stimulation. These results indicate that electrical stimulation-induced increase of dopamine is a nerve impulse exocytotic process and suggest that cortical dopamine and noradrenaline may be coreleased from noradrenergic terminals.