Simple sequence repeat-based comparative genomics between Brassica rapa and Arabidopsis thaliana:: The genetic origin of clubroot resistance
Simple sequence repeat-based comparative genomics between Brassica rapa and Arabidopsis thaliana:: The genetic origin of clubroot resistance
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DOI：
10.1534/genetics.104.038968
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发表时间：
2006-05-01
期刊：
影响因子：
3.3
通讯作者：
Matsumoto, Satoru
中科院分区：
文献类型：
--
作者：
Suwabe, Keita;Tsukazaki, Hikaru;Matsumoto, Satoru
构建了芜菁SSR分子标记连锁图谱。其中SSR标记113个，RFLP标记87个，RAPD标记62个。它由10个连锁群组成，总距离为1005.5cM，平均距离为3.7cM。SSR分布在整个连锁群中，平均为8.7 cM。B之间的同线性。分析了芜菁和模式植物拟南芥之间的关系。A.拟南芥散布在整个B。rapa连锁图谱这表明十字花科植物在进化过程中存在复杂的基因组重排。在B的282.5 cM区域。rapa map与A. thaliana.共线性分析表明，在抗根肿病的3个QTL（Crr1、Crr2和Crr4）中，Crr1和Crr2两个主要QTL在拟南芥4号染色体的一个小区域内重叠。该区域属于A.拟南芥基因组这些结果表明，根肿病的抗性基因起源于一个共同的祖先基因组中的一个成员的MRC，随后分布到不同的区域，他们现在居住在进化过程中。
An SSR-based linkage map was constructed in Brassica rapa. It includes 113 SSR, 87 RFLP, and 62 RAPD markers. It consists of 10 linkage groups with a total distance of 1005.5 cM and an average distance of 3.7 cM. SSRs are distributed throughout the linkage groups at an average of 8.7 cM. Synteny between B. rapa and a model plant, Arabidopsis thaliana, was analyzed. A number of small genomic segments of A. thaliana were scattered throughout an entire B. rapa linkage map. This points out the complex genomic rearrangements during the course of evolution in Cruciferae. A 282.5-cM region in the B. rapa map was in synteny with A. thaliana. Of the three QTL (Crr1, Crr2, and Crr4) for clubroot resistance identified, synteny analysis revealed that two major QTL rcgions, Crr1 and Crr2, overlapped in a small region of Arabidopsis chromosome 4. This region belongs to one of the disease-resistance gene clusters (MRCs) in the A. thaliana genome. These results suggest that the resistance genes for clubroot originated from a member of the MRCs in a common ancestral genome and subsequently were distributed to the different regions they now inhabit in the process of evolution.