Purification of a cultivar-specific toxin from Pyrenophora tritici-repentis, causal agent of tan spot of wheat.
Purification of a cultivar-specific toxin from Pyrenophora tritici-repentis, causal agent of tan spot of wheat.
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从小麦赤霉病菌（小麦褐斑病的致病因子）中纯化品种特异性毒素。
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
J. Leach
中科院分区：
文献类型：
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作者：
A. Tomas;G. Feng;G. Reeck;W. Bockus;J. Leach
小麦褐斑病病原菌——褐斑病菌（Pyrenophora tritrii -repentis）在培养过程中产生一种毒素，仅在对该病菌敏感的小麦品种上引起坏死。通过凝胶过滤层析和离子交换层析纯化了该有毒化合物，命名为Ptr毒素。与大多数其他真菌植物毒素不同，Ptr毒素是一种低分子量蛋白质，分子量为14,700。用二硫苏糖醇还原可以消除毒性活性，这表明存在半胱氨酸，活性需要二硫键
Pyrenophora tritici-repentis, causal agent of tan spot of wheat, produces a toxin in culture that induces necrosis only on wheat cultivars susceptible to the pathogen. The toxic compound, named Ptr toxin, was purified by gel filtration chromatography and ion exchange chromatography. Unlike most other fungal phytotoxins, Ptr toxin is a low molecular weight protein of 14,700. Reduction with dithiothreitol abolished toxic activity indicating that cysteine is present and that a disulfide bond is required for activity