De novo shoot organogenesis during plant regeneration.
De novo shoot organogenesis during plant regeneration.
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DOI：
10.1093/jxb/erz395
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发表时间：
2019
影响因子：
6.9
通讯作者：
Jinwoo Shin;S. Bae;P. Seo
中科院分区：
文献类型：
--
作者：
Jinwoo Shin;S. Bae;P. Seo
植物表现出卓越的再生能力，确保发育可塑性。体外组织培养技术基于植物的再生能力，有利于从外植体产生新器官甚至整株植物。植物体细胞可以重新编程形成称为愈伤组织的多能细胞团。一部分多能愈伤组织细胞通过从头芽器官发生（DNSO）产生可育芽。在这里，我们重建了芽再生过程的四个阶段，包括多能性获得、芽原分生组织形成、受限芽祖细胞的建立和芽生长。此外，还总结了其他生物过程，包括细胞周期进程和活性氧代谢，这些过程进一步有助于 DNSO 的成功完成。总体而言，本研究强调了 DNSO 涉及的分子和细胞事件以及 DNSO 关键步骤背后的调控机制的最新进展。
Plants exhibit remarkable regeneration capacity, ensuring developmental plasticity. In vitro tissue culture techniques are based on the plant regeneration ability and facilitate production of new organs and even whole plant from explants. Plant somatic cells can be reprogrammed to form a pluripotent cell mass called callus. A portion of pluripotent callus cells gives rise to a fertile shoot via de novo shoot organogenesis (DNSO). Here, we reconstitute the shoot regeneration process with four phases, including pluripotency acquisition, shoot promeristem formation, establishment of confined shoot progenitor, and shoot outgrowth. Additionally, other biological processes, including cell cycle progression and reactive oxygen species metabolism, which further contribute to successful completion of DNSO, are also summarized. Overall, this study highlights recent advances in the molecular and cellular events involved in DNSO and regulatory mechanisms behind key steps of DNSO.