Distribution of Selenium and Mercury in the Components of Skipjack and Red Sea Bream Tissues

Distribution of Selenium and Mercury in the Components of Skipjack and Red Sea Bream Tissues
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硒和汞在鲣鱼和红鲷组织成分中的分布

DOI:
10.3358/shokueishi.20.299
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发表时间:
1979
期刊:
Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)
影响因子:
--
通讯作者:
H. Akehashi
H. Akehashi
中科院分区:
--
文献类型:
--
作者:
K. Itano;K. Sasaki;H. Akehashi

文献摘要

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将鲣鱼(Katsuwonus pelamis)和红鲷鱼(Chrysophrys major)的组织分离为提取物(Ex)、肌浆蛋白(Sp-P)、肌纤维蛋白(f- p)和碱不溶性残留物(R) 4种组分,测定其汞和硒的含量。所使用的组织是鱼的普通肌肉和肝脏以及鲣鱼的深色肌肉。两鱼普通肌肉中的总汞主要存在于Mf-P和Sp-P部分,硒主要存在于蛋白质和Ex部分。鲣鱼肝脏中约80%的硒是在Ex中发现的。普通肌肉中总汞含量与Mf-P呈线性关系,Sp-P也有类似的趋势。mp - p中汞含量高于Sp-P。普通肌肉中硒的含量与添加磷酸盐缓冲液(Ex + Sp-P)的提取物中硒的含量也呈线性关系,而Mf-P中硒的含量也有类似的趋势。提取物中的硒含量与普通肌肉中的硒含量密切相关。还研究了半胱氨酸和半胱胺对两种鱼类普通肌肉中汞的去除作用。这两种化合物去除了不同比例的汞。
The tissues of skipjack (Katsuwonus pelamis) and red sea bream (Chrysophrys major) were fractionated into four components, extractives (Ex), sarcoplasmic protein (Sp-P), myofibrillar protein (Mf-P) and alkali-insoluble residue (R), to determine the contents of mercury and selenium in these components. The tissues used were the ordinary muscle and liver of both fishes and dark muscle of skipjack. The total mercury in the ordinary muscle of both fishes was present in the Mf-P and Sp-P fractions, while the selenium was contained in the protein and Ex fractions. About 80% of the selenium in the liver of skipjack was found in Ex. There was a linear relation between the total mercury content in ordinary muscle and that in Mf-P, and a similar tendency was observed in Sp-P. More mercury was incorporated in Mf-P than in Sp-P. There was also a linear relation between the selenium content in ordinary muscle and that in the extract with phosphate buffer (Ex plus Sp-P), and a similar tendency was observed in Mf-P. The selenium content in the extract depended strongly on the selenium content in ordinary muscle. Mercury removal from the ordinary muscle of both fishes by cysteine and cysteamine was also investigated. Different proportions of the mercury were removed by the two compounds.