Stromal cells in long-term cultures: keys to the elucidation of hematopoietic development?
Stromal cells in long-term cultures: keys to the elucidation of hematopoietic development?
复制标题
DOI：
--
复制
发表时间：
1993
影响因子：
1.3
通讯作者：
E. Deryugina;C. Müller-Sieburg
中科院分区：
文献类型：
--
作者：
E. Deryugina;C. Müller-Sieburg
长期骨髓培养反映了体内哺乳动物造血的许多方面。因此，这些培养物已被广泛用于分析调节造血分化的复杂相互作用。体内和体外的造血依赖于基质细胞，即支持细胞的混合物。在过去的几年中，许多克隆基质细胞系来源于小鼠和人类组织已被分离和表征。基质细胞系已被证明是阐明造血细胞分化和自我更新的分子和细胞信号的宝贵工具。本文综述了箭文化的显着特点，重点对基质细胞系的分离和表征。我们总结了基质细胞系如何成为检测、分离和维持稀有多能干细胞和B谱系细胞的重要工具。关于基质细胞的性质、其生长和分化的要求以及其组织发生起源的有趣问题仍有待进一步研究。
Long-term bone marrow cultures mirror many aspects of in vivo mammalian hematopoiesis. Thus, these cultures have been used widely to analyze the complex interactions that regulate hematopoietic differentiation. Hematopoiesis in vivo and in vitro is dependent on stromal cells, a mixture of support cells. In the past years numerous clonal stromal cell lines derived from murine and human tissues have been isolated and characterized. The stromal cell lines have proved to be invaluable tools for the elucidation of the molecular and cellular signals that govern differentiation and self-renewal of hematopoietic cells. This review describes the salient features of arrow cultures with a focus on the isolation and characterization of stromal cell lines. We summarize how stromal cell lines have been crucial tools for the detection, isolation, and maintenance of rare pluripotent stem cells and B lineage cells. Intriguing questions about the nature of stromal cells, their requirements for growth and differentiation, and their histogenic origin remain areas for further investigation.