Polymer Nanoreactors with Dual Functionality: Simultaneous Detoxification of Peroxynitrite and Oxygen Transport
Polymer Nanoreactors with Dual Functionality: Simultaneous Detoxification of Peroxynitrite and Oxygen Transport
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DOI：
10.1021/la302724m
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发表时间：
2012-11-13
期刊：
影响因子：
3.9
通讯作者：
Palivan, Cornelia G.
中科院分区：
文献类型：
--
作者：
Dobrunz, Dominik;Toma, Adriana C.;Palivan, Cornelia G.
多功能系统的设计是当今的焦点：是一个关键。应对化学、医学、环境科学和技术等各个领域复杂挑战的策略。在此，我们介绍了具有双重功能的含蛋白质聚合物纳米反应器：过氧亚硝酸盐降解和氧运输。由聚（2-甲基氯唑啉）-聚（二甲基硅氧烷）-聚（2-甲基氯唑啉）组成的囊泡成功地封装了血红蛋白（Hb），因为它具有氧转运和过氧亚硝酸盐降解的双重功能。通过插入通道蛋白，囊泡的聚合物膜允许各种化合物通过，用于评估原位Hb活性。必要的构象变化。在蛋白质中。结构和。在囊泡腔的有限空间内发生的氧化态变化表明Hb保留了其双重功能：过氧亚硝酸盐降解和氧运输。我们的纳米反应器的功能，结合其简单的生产过程和数月的广泛稳定性，支持它作为一个有前途的系统进一步的医疗应用。
The design of multifunctional systems is in focus today:is a key. strategy for coping with complex challenges in various domains that include chemistry, medicine, environmental sciences, and technology. Herein, we introduce protein-containing polymer nanoreactors with dual functionality: peroxynitrite degradation and oxygen transport. Vesicles made of poly-(2-methyloxazoline)-poly(dimethylsiloxane)-poly(2-methyloxazoline) successfully encapsulated hemoglobin (Hb), which serves as a model protein because of its dual function in oxygen transport and peroxynitrite degradation. By inserting channel proteins, the polymer membranes of vesicles permitted passage of various compounds that served for the assessment of in situ Hb activity. The requisite conformational changes. in the protein. structure and the. change in oxidation states that took place within the confined space of the vesicle cavity demonstrated that Hb preserved its dual functionality: peroxynitrite degradation and oxygen transport The functionality of our nanoreactor, combined With its simple procedure of production and extensive stability over several months, supports it as a promising system for further medical applications.