Ac transposition in transgenic tomato plants
Ac transposition in transgenic tomato plants
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转基因番茄植物中的 Ac 转座
DOI：
10.1007/bf00339594
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
M. Lassner
中科院分区：
文献类型：
--
作者：
J. Yoder;J. Palys;K. Alpert;M. Lassner
作为在番茄中开发转座子诱变系统的第一步，通过根癌农杆菌将玉米转座因子Ac转化到番茄植株中。叶片组织的Southern分析表明，在11个转基因植物中的9个中，Ac从T-DNA中切除并重新整合到新的染色体位置。同一初生植株不同叶片Ac带型的比较为转基因植株发育后期的转座提供了依据。番茄转化体中没有Ds动员的证据。
SummaryAs an initial step towards developing a transposon mutagenesis system in tomato, the maize transposable element Ac was transformed into tomato plants via Agrobacterium tumefaciens. Southern analysis of leaf tissue indicated that in nine out of eleven transgenic plants, Ac excised from the T-DNA and reintegrated into new chromosomal locations. The comparison of Ac banding pattern in different leaves of the same primary transformant provided evidnece for transposition during later stages of transgenic plant development. There was no evidence of Ds mobilization in tomato transformants.