Real-Time Tracking of BODIPY-C12 Long-Chain Fatty Acid in Human Term Placenta Reveals Unique Lipid Dynamics in Cytotrophoblast Cells.
Real-Time Tracking of BODIPY-C12 Long-Chain Fatty Acid in Human Term Placenta Reveals Unique Lipid Dynamics in Cytotrophoblast Cells.
复制标题
人体胎盘中Bodipy-C12长链脂肪酸的实时跟踪揭示了细胞增生细胞细胞中独特的脂质动力学。
DOI：
10.1371/journal.pone.0153522
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Thornburg K
中科院分区：
文献类型：
--
作者：
Kolahi K;Louey S;Varlamov O;Thornburg K
虽然人类胎盘必须提供选择的长链脂肪酸来支持胎儿大脑的发育，但对运输过程的潜在机制知之甚少。我们跟踪了荧光标记的长链脂肪酸类似物BODIPY-C12在人足月胎盘活外植体细胞层中的运动。虽然所有层都吸收脂肪酸，但长链脂肪酸的快速酯化和脂滴掺入是内层细胞滋养层细胞所独有的，而不是预期的外层合胞滋养层。细胞滋养细胞是一种祖细胞层，以前被降级为修复作用。当分离的细胞滋养细胞在培养中分化为合胞细胞时，它们的脂质处理能力减弱。合胞细胞抑制了先前活跃的调节脂肪酸摄取（SLC27A2/FATP2, FABP4, ACSL5）和脂质代谢（GPAT3, LPCAT3）的基因。我们推测细胞滋养细胞在调节胎盘脂肪酸摄取和代谢中发挥了以前未被认识到的作用。
While the human placenta must provide selected long-chain fatty acids to support the developing fetal brain, little is known about the mechanisms underlying the transport process. We tracked the movement of the fluorescently labeled long-chain fatty acid analogue, BODIPY-C12, across the cell layers of living explants of human term placenta. Although all layers took up the fatty acid, rapid esterification of long-chain fatty acids and incorporation into lipid droplets was exclusive to the inner layer cytotrophoblast cells rather than the expected outer syncytiotrophoblast layer. Cytotrophoblast is a progenitor cell layer previously relegated to a repair role. As isolated cytotrophoblasts differentiated into syncytialized cells in culture, they weakened their lipid processing capacity. Syncytializing cells suppress previously active genes that regulate fatty-acid uptake (SLC27A2/FATP2, FABP4, ACSL5) and lipid metabolism (GPAT3, LPCAT3). We speculate that cytotrophoblast performs a previously unrecognized role in regulating placental fatty acid uptake and metabolism.