Bioinformatic analyses of the publicly accessible crustacean expressed sequence tags (ESTs) reveal numerous novel neuropeptide-encoding precursor proteins, including ones from members of several little studied taxa
Bioinformatic analyses of the publicly accessible crustacean expressed sequence tags (ESTs) reveal numerous novel neuropeptide-encoding precursor proteins, including ones from members of several little studied taxa
复制标题
DOI：
10.1016/j.ygcen.2010.01.005
复制
发表时间：
2010-05-15
影响因子：
2.7
通讯作者：
Lenz, Petra H.
中科院分区：
文献类型：
--
作者：
Christie, Andrew E.;Durkin, Christopher S.;Lenz, Petra H.
许多甲壳类动物的EST已经产生，为在这个节肢动物亚门的成员中发现肽提供了宝贵的资源。在这里，这些数据被挖掘为新的肽编码转录本，与成熟的肽编码的预测使用的组合，在线肽预测程序和同源性已知的节肢动物序列。总共预测了代表16个家族/亚家族成员的70个成熟的全长/部分肽，绝大多数是新的;鉴定出肽的物种包括鳃足类的成员（隆线水蚤和癌症状三眼水蚤），颚足类（Caligus clemensi、Caligus rogercresseyi、Lepeophtheirus salmonis和Lernaeocera branchialis）和软甲藻（Euphausia superba、Marsupenaeus spanish、Penaeus monodon、Homarus americanus、Petrolithes cinctipes、Callinectes sapidus和Portunus trituberculatus）。特别值得注意的是鉴定了昆虫脂肪运动激素和甲壳动物红色素浓缩激素之间的中间体和来自水蚤D的修饰的甲壳动物心脏活性肽。carinata：Arg（7）-corazonin也从该物种中推导出来，这是首次从非十足类甲壳动物中鉴定出corazonin。我们的数据还包括降钙素样利尿激素，FMRFamide相关肽（神经肽F亚家族）和orcokinin家族成员的桡足类的第一次报告。此外，对磷虾E. Superb代表了从Euphausiacea的基底Euphausiacea的任何成员中鉴定的第一个肽。此外，大量收集的昆虫羽化激素和neuroparsin-like肽被确定从各种物种，大大扩大了这些家庭的甲壳类动物的已知成员的数量。(C)2010年爱思唯尔公司All rights reserved.
ESTs have been generated for many crustacean species, providing an invaluable resource for peptide discovery in members of this arthropod subphylum. Here, these data were mined for novel peptide-encoding transcripts, with the mature peptides encoded by them predicted using a combination of online peptide prediction programs and homology to known arthropod sequences. In total, 70 mature full-length/partial peptides representing members of 16 families/subfamilies were predicted, the vast majority being novel; the species from which the peptides were identified included members of the Branchiopoda (Daphnia carinata and Triops cancriformis), Maxillopoda (Caligus clemensi, Caligus rogercresseyi, Lepeophtheirus salmonis and Lernaeocera branchialis) and Malacostraca (Euphausia superba, Marsupenaeus japonicus, Penaeus monodon, Homarus americanus, Petrolisthes cinctipes, Callinectes sapidus and Portunus trituberculatus). Of particular note were the identifications of an intermediate between the insect adipokinetic hormones and crustacean red pigment concentrating hormone and a modified crustacean cardioactive peptide from the daphnid D. carinata: Arg(7)-corazonin was also deduced from this species, the first identification of a corazonin from a non-decapod crustacean. Our data also include the first reports of members of the calcitonin-like diuretic hormone, FMRFamide-related peptide (neuropeptide F subfamily) and orcokinin families from members of the Copepoda. Moreover, the prediction of a bursicon alpha from the euphausid E. superb represents the first peptide identified from any member of the basal eucaridean order Euphausiacea. In addition, large collections of insect eclosion hormone- and neuroparsin-like peptides were identified from a variety of species, greatly expanding the number of known members of these families in crustaceans. (C) 2010 Elsevier Inc. All rights reserved.