Synchronous in-field application of life-detection techniques in planetary analog missions
Synchronous in-field application of life-detection techniques in planetary analog missions
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DOI：
10.1016/j.pss.2014.11.006
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发表时间：
2015-02-01
影响因子：
2.4
通讯作者：
Geppert, Wolf
中科院分区：
文献类型：
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作者：
Amador, Elena S.;Cable, Morgan L.;Geppert, Wolf
在地球上的模拟地点模拟机器人行星探索任务操作的实地考察可以帮助建立最佳实践，因此对行星探索界做出了积极贡献。冰岛有许多地点拥有行星探索模拟地点的遗产，其极端环境使其适合模拟科学采样和机器人操作。我们使用专门开发的现场实验室，在冰岛最近的两个熔岩场 Fimmvorouhals (2010) 和 Eldfell (1973) 进行了行星探索模拟任务。我们通过三种生命检测和表征技术测试了现场现场采样向下选择和分层分析操作能力的实用性：荧光显微镜 (FM)、三磷酸腺嘌呤 (ATP) 生物发光测定和定量聚合酶链反应 (qPCR) 测定。该研究利用多个距离尺度的样本采集周期和使用同步法夫检测技术的现场实验室分析，启发性地制定了探险期间的持续采样和分析策略。在这里，我们报告了所吸取的操作经验教训，并提供了科学数据的简要总结。完整的科学数据报告将另行发布。我们发现，通过快速现场分析来确定后续采样决策在操作上是可行的，并且所选择的生命探测和表征技术适合陆地生命探测现场任务。现场分析可以快速获取科学数据，从而有助于在单次实地考察中收集最科学相关的样本，而无需将样本转移到外部实验室。这项研究中学到的操作经验教训可以应用于未来采用其他分析技术的陆地野外探险以及未来的机器人行星探索任务。 (C) 2014 Elsevier Ltd. 保留所有权利。
Field expeditions that simulate the operations of robotic planetary exploration missions at analog sites on Earth can help establish best practices and are therefore a positive contribution to the planetary exploration community. There are many sites in Iceland that possess heritage as planetary exploration analog locations and whose environmental extremes make them suitable for simulating scientific sampling and robotic operations.We conducted a planetary exploration analog mission at two recent lava fields in Iceland, Fimmvorouhals (2010) and Eldfell (1973), using a specially developed field laboratory. We tested the utility of in-field site sampling down selection and tiered analysis operational capabilities with three life detection and characterization techniques: fluorescence microscopy (FM), adenine-triphosphate (ATP) bioluminescence assay, and quantitative polymerase chain reaction (qPCR) assay. The study made use of multiple cycles of sample collection at multiple distance scales and field laboratory analysis using the synchronous fife-detection techniques to heuristically develop the continuing sampling and analysis strategy during the expedition.Here we report the operational lessons learned and provide brief summaries of scientific data. The full scientific data report will follow separately. We found that rapid in-field analysis to determine subsequent sampling decisions is operationally feasible, and that the chosen life detection and characterization techniques are suitable for a terrestrial life-detection field mission.In-field analysis enables the rapid obtainment of scientific data and thus facilitates the collection of the most scientifically relevant samples within a single field expedition, without the need for sample relocation to external laboratories. The operational lessons learned in this study could be applied to future terrestrial field expeditions employing other analytical techniques and to future robotic planetary exploration missions. (C) 2014 Elsevier Ltd. All rights reserved.