Simple method for determination of the number of Helicobacter pylori CagA variable-region EPIYA tyrosine phosphorylation motifs by PCR
Simple method for determination of the number of Helicobacter pylori CagA variable-region EPIYA tyrosine phosphorylation motifs by PCR
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DOI：
10.1128/jcm.43.2.791-795.2005
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发表时间：
2005-02-01
影响因子：
9.4
通讯作者：
Atherton, JC
中科院分区：
文献类型：
--
作者：
Argent, RH;Zhang, YL;Atherton, JC
具有cag致病岛的幽门螺杆菌与胃癌的发生有关。CagA蛋白易位到上皮细胞中，并在EPIYA基序内的酪氨酸残基上磷酸化，其可以在蛋白质的可变区内重复。具有这些重复的更大数量的CagA的菌株与胃癌发生更密切相关。磷酸化CagA导致上皮细胞伸长，这取决于可变区EPIYA基序的数量。因此，确定CagA磷酸化的程度和EPIYA基序的数量似乎比单独检测cagA更重要。然而，通过核苷酸测序确定EPIYA基序的数量是一个费力且昂贵的过程。我们在这里描述了一种新的和快速的PCR方法，用于确定的模式的重复含有EPIYA基序。这将有助于识别那些可能更有可能导致疾病的菌株。
Helicobacter pylori strains possessing the cag pathogenicity island are associated with the development of gastric cancer. The CagA protein is translocated into epithelial cells and becomes phosphorylated on tyrosine residues within EPIYA motifs, which may be repeated within the variable region of the protein. Strains possessing CagA with greater numbers of these repeats have been more closely associated with gastric carcinogenesis. Phosphorylated CagA leads to epithelial cell elongation, which is dependent on the number of variable-region EPIYA motifs. Thus, determination of the degree of CagA phosphorylation and the number of EPIYA motifs appears to be more important than detection of cagA alone. Determination of the number of EPIYA motifs by nucleotide sequencing, however, is a laborious and expensive process. We describe here a novel and rapid PCR method for determination of the pattern of repeats containing the EPIYA motif. This will aid in the identification of those strains that may be more likely to cause disease.