Pre-conceptional natural killer cell activity and percentage as predictors of biochemical pregnancy and spontaneous abortion with normal chromosome karyotype
Pre-conceptional natural killer cell activity and percentage as predictors of biochemical pregnancy and spontaneous abortion with normal chromosome karyotype
复制标题
DOI：
10.1034/j.1600-0897.2003.00095.x
复制
发表时间：
2003-10-01
影响因子：
3.6
通讯作者：
Minakami, H
中科院分区：
文献类型：
--
作者：
Yamada, H;Morikawa, M;Minakami, H
问题：本研究的目的是确定受孕前自然杀伤 (NK) 细胞活性和百分比是否可以预测患有复发性自然流产 (RSA) 的女性随后的自然流产。研究方法：对有两种或两种以上 RSA 病史的女性的受孕前自然杀伤 (NK) 细胞活性和外周血百分比进行前瞻性评估。进行 Cr-51 释放测定和流式细胞术分析。总共招募了 113 名 RSA 女性，其中 85 名后来受孕。 结果：下一次妊娠以生化妊娠和胎儿核型正常自然流产结束的女性的受孕前 NK 细胞活性/百分比值（n = 17，中位数 47%/17.1%），但异常核型自然流产（n = 9，27%/15.7%）， 高于活产婴儿 (n = 59, 33%/13.1%)。研究发现，孕前 NK 细胞活性的高值（> 46%；相对风险 3.6，95% CI 1.6-8.0）和百分比（> 16.4%；4.9，1.7-13.8）可预测下次妊娠时生化妊娠和核型正常的自然流产。 结论：孕前 NK 细胞异常可预测正常胎儿的自然流产 核型。
PROBLEM: The aim of the present study was to determine whether pre-conceptional natural killer (NK) cell activity and percentage are predictive of subsequent spontaneous abortion in women with recurrent spontaneous abortion (RSA).METHOD OF STUDY: Pre-conceptional NK cell activity and percentage in peripheral blood of women who had a history of two or more RSA was prospectively assessed. The Cr-51 release assay and flow cytometric analysis were performed. A total of 113 RSA women were recruited, and 85 conceived later.RESULTS: Pre-conceptional NK cell activity/percentage values in women whose next pregnancies ended in biochemical pregnancy and spontaneous abortion with normal fetal karyotype (n = 17, median 47%/17.1%), but spontaneous abortion with abnormal karyotype (n = 9, 27%/15.7%), were higher than those in live births (n = 59, 33%/13.1%). High values of pre-conceptional NK cell activity ( > 46%; relative risk 3.6, 95%CI 1.6-8.0) and percentage ( > 16.4%; 4.9, 1.7-13.8) were found to predict biochemical pregnancy and spontaneous abortion with normal karyotype in the next pregnancy.CONCLUSION: Pre-conceptional NK cell abnormalities were predictive of spontaneous abortion with normal fetal karyotype.