Immunogenicity of two Echinococcus granulosus antigens EgA31 and EgTrp in mice
Immunogenicity of two Echinococcus granulosus antigens EgA31 and EgTrp in mice
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DOI：
10.1007/s00436-005-1322-x
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发表时间：
2005-05-01
影响因子：
2
通讯作者：
Petavy, AF
中科院分区：
文献类型：
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作者：
Fraize, M;Sarciron, ME;Petavy, AF
在此，我们研究了细粒棘球绦虫幼虫阶段表达的两种抗原EgA31和EgTrp在小鼠体内的免疫原性。将这些重组蛋白单独或作为混合物(EgA31-EgTrp)免疫BALB/c小鼠。流式细胞仪检测显示，抗原免疫组小鼠脾细胞中CD4+/CD8+比值明显升高。用酶联免疫吸附试验检测血清中的特异性抗体和产生细胞因子的脾细胞。EgA31、EgTrp或EgA31-EgTrp可诱导高滴度的抗体滴度。在免疫球蛋白亚型中，单独检测或联合检测的每一种抗原均以IgG1为主。重组蛋白免疫小鼠后，IL-12、干扰素-γ、IL-10和IL-6细胞因子的产生明显增加。我们的结果表明，在BALB/c小鼠中，获得了对EgA31、EgTrp和EgA31-EgTrp的混合Th1/Th2反应。讨论了将这两种抗原单独或组合作为候选疫苗蛋白的使用。
Here, we investigate in mice the immunogenicity of two antigens EgA31 and EgTrp which are expressed by the larval stage of Echinococcus granulosus. These recombinant proteins were used alone or as a mixture ( EgA31 - EgTrp) to immunize BALB/c mice. By flow cytometry, we have shown that the ratio CD4+/ CD8+ of splenocytes were significantly higher in the antigen-immunized groups. The specific antibody in the sera and cytokine producing splenocytes was evaluated by enzyme-linked immunosorbent assay. EgA31, EgTrp or EgA31 - EgTrp elicited high antibody titer of IgG and IgA. Among IgG isotypes, IgG1 was predominant for each antigen tested alone or combined. The production of IL-12, IFN-gamma, IL-10 and IL-6 cytokines was significantly higher in mice immunized with recombinant proteins. Our results suggest that, in BALB/c mice, a mixed Th1/Th2, response to EgA31, EgTrp and EgA31 - EgTrp is obtained. The use of both antigens separately or in combination as candidate vaccine proteins is discussed.