Experimental Stroke in Gerbils: Correlation of Clinical, Pathological and Electroencephalographic Findings and Protein Synthesis
Experimental Stroke in Gerbils: Correlation of Clinical, Pathological and Electroencephalographic Findings and Protein Synthesis
复制标题
沙鼠实验性中风：临床、病理和脑电图结果与蛋白质合成的相关性
DOI：
10.1161/01.str.9.2.155
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发表时间：
1978
期刊：
影响因子：
8.3
通讯作者：
T. Yanagihara
中科院分区：
文献类型：
--
作者：
T. Yanagihara
通过结扎右颈总动脉在沙鼠中产生脑缺血，并从30分钟至24小时跟踪所产生的临床表现和病理改变，沿着脑电图结果。用体外脑切片和随后的细胞和亚细胞分级来评价蛋白质合成。一组动物迅速出现脑缺血和中风的临床症状，通常在12小时内死亡。在这些动物中，脑梗死在术后几小时内弥漫在右侧大脑中，并且脑电图记录中存在持续抑制。亚细胞组分的蛋白质中的氨基酸掺入在30分钟时减少到对侧的50%，并且在8至10小时内进一步减少到小于10%。另一组动物存活至24小时，尽管有严重的神经系统表现，蛋白质合成在24小时时约为对照侧的15%。在神经元和神经胶质组分中均观察到蛋白质合成的抑制，表明这些细胞成分对脑缺血的脆弱性与过去脑缺氧所示的相似。强调了临床表现和生化数据的相关性对于从该模型中的生化研究中提取有意义的信息是非常重要的。
Cerebral ischemia was produced in gerbils by ligation of the right common carotid artery and the resulting clinical manifestations and pathological alterations, along with electroencephalographic findings, were followed from 30 minutes to 24 hours. Protein synthesis was evaluated with brain slices in vitro and subsequent cellular and subcellular fractionations. One group of animals developed clinical signs of cerebral ischemia and stroke very rapidly and often died within 12 hours. In these animals cerebral infarction was diffuse in the right side of brain within a few hours postoperatively and there was persistent suppression in the electroencephalographic recordings. Amino acid incorporation into proteins of subcellular fractions was decreased to 50% of the opposite side at 30 minutes and further declined to less than 10% in 8 to 10 hours. Another group of animals survived to 24 hours in spite of severe neurological manifestations, and protein synthesis was about 15% of the control side at 24 hours. The suppression of protein synthesis was observed both in the neuronal and neuroglial fractions indicating similar vulnerability of these cellular elements toward cerebral ischemia as shown with cerebral anoxia in the past. It was emphasized that the correlation of clinical manifestations and biochemical data is very important to extract meaningful information from biochemical investigations in this model.