Latex immunoassay of human serum Lp(a+) lipoprotein.
Latex immunoassay of human serum Lp(a+) lipoprotein.
复制标题
人血清 Lp(a) 脂蛋白的乳胶免疫测定。
DOI：
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发表时间：
1985
影响因子：
6.5
通讯作者：
J. Fruchart
中科院分区：
文献类型：
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作者：
N. Vu;A. Chekkor;H. Parra;P. Duthilleul;J. Fruchart
人血清脂蛋白Lp(a+)的灵敏胶乳免疫分析是基于Lp(a+)与包被特异性抗体的胶乳颗粒的直接凝集。凝集作用用360 nm处的光度计比浊法定量。牛血清白蛋白对包被抗体的乳胶粒子的稳定作用发生在明确的条件下(pH、牛血清白蛋白浓度和乳胶粒子的抗体载量)。血清脂蛋白Lp(a+)的标准曲线范围为0.0 5~1.15 mg/L，批内和批间变异系数分别小于8%和3%。结果与免疫扩散法检测结果具有良好的相关性(r=0.98，n=108)。
A sensitive latex immunoassay for human serum lipoprotein Lp(a+) is based on direct agglutination by Lp(a+) of latex particles coated with specific antibody. The agglutination is quantified by turbidimetry using a photometer at 360 nm. The stabilization of antibody-coated latex particles by bovine serum albumin occurs under well-defined conditions (pH, concentration of bovine serum albumin, and antibody loading of latex particles). The standard curve of serum lipoprotein Lp(a+) ranges from 0.05 to 1.15 mg/l. Inter- and intra-assay coefficients of variation were less than 8% and 3%, respectively. Results were well correlated with those obtained by electroimmunodiffusion (r = 0.98, n = 108).