Mucosally-directed adrenergic nerves and sympathomimetic drugs enhance non-intimate adherence of Escherichia coli O157:H7 to porcine cecum and colon
Mucosally-directed adrenergic nerves and sympathomimetic drugs enhance non-intimate adherence of Escherichia coli O157:H7 to porcine cecum and colon
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DOI：
10.1016/j.ejphar.2006.03.081
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发表时间：
2006-06-06
影响因子：
5
通讯作者：
Brown, David R.
中科院分区：
文献类型：
--
作者：
Chen, Chunsheng;Lyte, Mark;Brown, David R.
交感神经递质去甲肾上腺素已被发现增加肠出血性大肠杆菌O 157：H7在小鼠盲肠和猪远端结肠外植体的粘膜粘附。在本研究中，我们测试的假设，即去甲肾上腺素增强初始，松散的粘附这种重要的病原体肠粘膜。在猪盲肠或近端，螺旋和远端结肠的粘膜片安装在Ussing室，去甲肾上腺素（10 μ M，contraluminal添加）增加野生型E。大肠杆菌O 157：H7菌株85-170;在盲肠粘膜中，这种作用发生在细菌接种后30-90分钟内。此外，去甲肾上腺素短暂增加盲肠和结肠粘膜片的短路电流，这是一种主动阴离子转运的测量方法。去甲肾上腺素可有效促进非O 157 E的盲肠粘附。coli菌株以及E. coli O 157：H7 eae或espA突变株不能紧密粘膜附着。去甲肾上腺素合成酶多巴胺羟化酶免疫反应性神经纤维出现在盲肠上皮附近，去甲肾上腺素再摄取阻断剂可卡因，如去甲肾上腺素和选择性α（2）-肾上腺素受体激动剂UK-14，304，增加E。coli O 157：H7粘附。这些结果表明，去甲肾上腺素，作用于大肠粘膜，调节早期，非亲密的粘附E。大肠杆菌O 157：H7和可能的其他粘膜相关细菌。支配盲肠结肠粘膜的交感神经可能将急性应激暴露或精神兴奋剂滥用与肠道表面微生物定植增加联系起来。这反过来又可能改变宿主对肠道感染的易感性。(c)2006 Elsevier B. V.保留所有权利。
The sympathetic neurotransmitter norepinephrine has been found to increase mucosal adherence of enterohemorrhagic Escherichia coli O157: H7 in explants of murine cecum and porcine distal colon. In the present study, we tested the hypothesis that norepinephrine augments the initial, loose adherence of this important pathogen to the intestinal mucosa. In mucosal sheets of porcine cecum or proximal, spiral and distal colon mounted in Ussing chambers, norepinephrine (10 mu M, contraluminal addition) increased mucosal adherence of wild-type E. coli O157:H7 strain 85-170; in the cecal mucosa, this effect occurred within 30-90 min after bacterial inoculation. In addition, norepinephrine transiently increased short-circuit current in cecal and colonic mucosal sheets, a measure of active anion transport. Norepinephrine was effective in promoting cecal adherence of a non-O157 E. coli strain as well as E. coli O157:H7 eae or espA mutant strains that are incapable of intimate mucosal attachment. Nerve fibers immunoreactive for the norepinephrine synthetic enzyme dopamine -hydroxylase appeared in close proximity to the cecal epithelium, and the norepinephrine reuptake blocker cocaine, like norepinephrine and the selective alpha(2)-adrenoceptor agonist UK-14,304, increased E. coli O157:H7 adherence. These results suggest that norepinephrine, acting upon the large bowel mucosa, modulates early, non-intimate adherence of E. coli O157:H7 and probably other mucosa-associated bacteria. Sympathetic nerves innervating the cecocolonic mucosa may link acute stress exposure or psychostimulant abuse with an increased microbial colonization of the intestinal surface. This in turn may alter host susceptibility to enteric infections. (c) 2006 Elsevier B.V. All rights reserved.