Iron-reducing capacity of low-molecular-weight compounds produced in wood by fungi
Iron-reducing capacity of low-molecular-weight compounds produced in wood by fungi
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DOI：
10.1515/hf.2006.106
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发表时间：
2006-01-01
期刊：
影响因子：
2.4
通讯作者：
Qian, Yuhui
中科院分区：
文献类型：
--
作者：
Goodell, Barry;Daniel, Geoffrey;Qian, Yuhui
从桦木和松材中分离到12株褐腐菌、26株白腐菌、6株软腐菌和4株蓝腐菌。均质木材随后在75%乙酸乙酯中提取并离心。对过滤后的提取物进行了铁还原能力的分析，采用了基于铁的分析方法。琼脂真菌培养也直接使用铁还原的现场测试进行检查。被褐腐菌侵染的木材提取物表现出比被白腐菌或非腐烂真菌侵染的木材提取物更强的铁还原能力。斑点试验的结果变化很大，但总的来说，最大的颜色反应与褐腐病培养有关。褐腐菌产生还原铁的化合物和/或修饰木材成分的能力与被认为是褐腐菌非酶降解木材的“螯合剂介导的Fenton机制”有关。
Birch and pine wood specimens were colonized by individual isolates of 12 brown-rot, 26 white-rot, six soft-rot and four blue (sap)-stain fungi. Homogenized wood was subsequently extracted in 75% ethyl acetate and centrifuged. The filtered extracts were analyzed for their iron-reducing capabilities using a ferrozine-based assay. Agar fungal cultures were also examined directly using a spot test for iron reduction. Extracts from wood colonized by brown-rot fungi showed significantly greater iron-reducing capability than extracts from wood colonized by white-rot or non-decay fungi. Results of the spot test ratings were highly variable, but in general the greatest color responses were associated with the brown-rot cultures. The ability of brown-rot fungi to produce compounds and/or modify the wood components that reduce iron is of relevance to the "chelator-mediated Fenton mechanism" that has been advanced as a theory for the nonenzymatic degradation of wood by brown-rot fungi.