Comparison of the gut microbial community between obese and lean peoples using 16S gene sequencing in a Japanese population.
Comparison of the gut microbial community between obese and lean peoples using 16S gene sequencing in a Japanese population.
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DOI：
10.3164/jcbn.15-152
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发表时间：
2016-07
影响因子：
2.4
通讯作者：
Kobayashi T
中科院分区：
文献类型：
--
作者：
Andoh A;Nishida A;Takahashi K;Inatomi O;Imaeda H;Bamba S;Kito K;Sugimoto M;Kobayashi T
肠道微生物生态的改变有助于包括肥胖在内的代谢性疾病的发展。在这项研究中，我们对肥胖和瘦弱的日本人群的肠道微生物群进行了16S rRNA序列分析。使用Illumina MiSeqTMII系统对10名肥胖和10名瘦弱志愿者粪便样本的16S rRNA的V3-V4高变区进行测序。肥胖组和瘦肉组的平均体重指数分别为38.1和16.6 kg/m2 （p<0.01）。瘦肉组Shannon多样性指数显著高于肥胖组（p<0.01）。肥胖人群中厚壁菌门和梭杆菌门的数量明显多于瘦人。拟杆菌门丰度和拟杆菌门/厚壁菌门比值在肥胖组和瘦组之间无显著差异。肥胖人群中Alistipes属、厌氧球菌属、Corpococcus属、Fusobacterium属和Parvimonas属的数量显著增加，瘦子人群中Bacteroides属、Desulfovibrio属、Faecalibacterium属、Lachnoanaerobaculum属和Olsenella属的数量显著增加。具有抗炎特性的细菌种类，如prausnitzii粪杆菌，在瘦人体内显著增加，但具有促炎特性的细菌种类在肥胖人群中增加。日本人群中与肥胖相关的肠道微生物群与西方人不同。
Altered gut microbial ecology contributes to the development of metabolic diseases including obesity. In this study, we performed 16S rRNA sequence analysis of the gut microbiota profiles of obese and lean Japanese populations. The V3–V4 hypervariable regions of 16S rRNA of fecal samples from 10 obese and 10 lean volunteers were sequenced using the Illumina MiSeqTMII system. The average body mass index of the obese and lean group were 38.1 and 16.6 kg/m2, respectively (p<0.01). The Shannon diversity index was significantly higher in the lean group than in the obese group (p<0.01). The phyla Firmicutes and Fusobacteria were significantly more abundant in obese people than in lean people. The abundance of the phylum Bacteroidetes and the Bacteroidetes/Firmicutes ratio were not different between the obese and lean groups. The genera Alistipes, Anaerococcus, Corpococcus, Fusobacterium and Parvimonas increased significantly in obese people, and the genera Bacteroides, Desulfovibrio, Faecalibacterium, Lachnoanaerobaculum and Olsenella increased significantly in lean people. Bacteria species possessing anti-inflammatory properties, such as Faecalibacterium prausnitzii, increased significantly in lean people, but bacteria species possessing pro-inflammatory properties increased in obese people. Obesity-associated gut microbiota in the Japanese population was different from that in Western people.