Peripheral nerve regeneration through allografts compared with autografts in FK506-treated monkeys
Peripheral nerve regeneration through allografts compared with autografts in FK506-treated monkeys
复制标题
DOI：
10.3171/jns.2006.105.4.602
复制
发表时间：
2006-10-01
影响因子：
4.1
通讯作者：
Gorria, Oscar
中科院分区：
文献类型：
--
作者：
Auba, Cristina;Hontanilla, Bernardo;Gorria, Oscar
物体。同种异体神经移植结合免疫抑制疗法的临床应用已经成为一种真正的可能性，可以取代经典的自体移植。然而，关于免疫抑制治疗是否应该暂时实施，有相互矛盾的数据报道。本研究的目的是比较使用FK506(他克莫司)临时处理的非人灵长类动物的同种异体尺神经移植和使用自体神经移植获得的神经再生。将4厘米长的自体或同种异体神经移植物植入8只猴的尺侧运动神经远端。同种异体移植组动物暂时给予FK506治疗2个月。术后3、5、8个月分别进行定量电生理记录，以评估肌肉反应。对脊髓内尺运动神经元进行了定量分析，并对轴突进行了立体计数，8个月时自体移植组和同种异体移植组之间的神经元和轴突计数差异无统计学意义。电生理检查显示波幅无差异，但自体移植组神经传导速度(NCV)较大。然而，两组神经元数量和远端轴突计数之间的差异无统计学意义。在用FK506临时处理的灵长类动物模型中，在神经元和轴突计数方面，通过冷保存的同种异体神经移植再生的神经与使用自体神经移植获得的神经再生相似。然而，当使用同种异体移植时，暂时的免疫抑制会产生较低的NCV，有髓纤维成熟程度较低，这表明已经发生了部分排斥反应。
Object. The clinical use of nerve allografts combined with immunosuppressant therapy has become a genuine possibility that could supersede the classic use of autografts. However, contradictory data have been reported on whether immunosuppressant therapy should be temporarily administered. The purpose of this study was to compare the nerve regeneration obtained using ulnar nerve allografts in nonhuman primates temporarily treated with FK506 (tacrolimus) with that obtained using nerve autografts.Methods. Four-centimeter nerve autografts or allografts were placed in the distal ulnar motor nerve of eight monkeys. The FK506 was temporarily administered to the animals of the allograft group for 2 months. At periods of 3, 5, and 8 months postsurgery, quantitative electrophysiological recordings were obtained to estimate muscle response. A quantitative analysis of ulnar motor neurons in the spinal cord was performed and axons were counted stereologically.No statistically significant differences were found in the neuronal and axonal counts between autograft and allograft groups at 8 months. The electrophysiological studies showed no differences relative to the amplitude, but the autograft group presented with a greater nerve conduction velocity (NCV). However, no statistically significant differences were found between the number of neurons and distal axonal counts in the two groups.Conclusions. Nerve regeneration through cold-preserved allografts in a primate model temporarily treated with FK506 was similar to that obtained using nerve autografts, in terms of neuronal and axonal counts. Nevertheless, temporary immunosuppression produced lower NCV when allografts were used, with less maturation of the myelinated fibers, which indicated that a partial rejection had taken place.