Defects in hemostasis in P-selectin-deficient mice
Defects in hemostasis in P-selectin-deficient mice
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DOI：
10.1182/blood.v87.4.1238.bloodjournal8741238
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发表时间：
1996-02-15
期刊：
影响因子：
20.3
通讯作者：
Wagner, DD
中科院分区：
文献类型：
--
作者：
Subramaniam, M;Frenette, PS;Wagner, DD
最近，我们的实验室显示，血小板，像白细胞一样，在表达P-选择素的活化内皮上滚动，从而表明P-选择素在止血中的作用（Frenette等，Proc Natl Acad Sci USA 92：7450，1995）。我们在这里报告说，P-选择素缺陷的小鼠表现出40%的延长出血时间的尾巴尖截肢。此外，在P-选择素缺陷小鼠中，在皮肤注射脂多糖后再注射肿瘤坏死因子-α诱导的局部Shwartzman样反应中观察到止血缺陷。在P-选择素缺陷小鼠中，肉眼和显微镜下定量的出血性病变大两倍。这也通过使用铬标记的红细胞测量皮肤中的放射性来证实。因此，很明显，P-选择素在止血中起作用，如其支持血小板滚动所示。(C)1996年，美国血液学会。
Recently, our laboratory showed that platelets, like leukocytes, roll on activated endothelium expressing P-selectin, thus suggesting a role for P-selectin in hemostasis (Frenette et al, Proc Natl Acad Sci USA 92:7450, 1995). We report here that the P-selectin-deficient mice show a 40% prolongation of the bleeding time on amputation of the tip of the tail. Moreover, defective hemostasis was observed in a local Shwartzman-like reaction induced by skin injections of lipopolysaccharide followed by tumor necrosis factor-alpha in the P-selectin-deficient mice. The hemorrhagic lesions, quantitated both macroscopically and microscopically, were twofold larger in the P-selectin-deficient mice. This was also confirmed by measuring the radioactivity in the skin using chromium-labeled red blood cells, Therefore, it is evident that P-selectin plays a role in hemostasis as suggested by its support of platelet rolling. (C) 1996 by The American Society of Hematology.