Site-selective reactions of imperfectly matched DNA with small chemical molecules: applications in mutation detection
Site-selective reactions of imperfectly matched DNA with small chemical molecules: applications in mutation detection
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DOI：
10.1016/s0045-2068(02)00019-6
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发表时间：
2002-08-01
影响因子：
5.1
通讯作者：
Cotton, RGH
中科院分区：
文献类型：
--
作者：
Bui, CT;Rees, K;Cotton, RGH
在过去的十年里，我们看到了许多令人兴奋的科学出版物，这些出版物与小化学分子与不完全匹配的DNA的位点选择性反应有关。典型的例子是碳二亚胺、羟胺、高锰酸钾、四氧化锇、化学标记探针、生物素化的荧光和荧光探针，它们都选择性地与不完全匹配的DNA反应。最近，一些治疗药物，包括DNA嵌入药物和沟槽结合剂，已被发现可以促进体内修复系统更有效地识别和修复错配。这一结果为失配检测提供了一种新的方法。开发新的化学反应检测不完全匹配的DNA和突变是一个快速增长的领域，并吸引了来自化学和生物领域的科学家的显着兴趣，这是本文的主要重点。(C)2002 Elsevier Science（美国）。All rights reserved.
The last decade has witnessed many exciting scientific publications associated with site-selective reactions of small chemical molecules with imperfectly matched DNA. Typical examples are carbodiimide, hydroxylamine, potassium permanganate, osmium tetroxide, chemical tagging probes, biotinylated, chemiluminescent and fluorescent probes, and all of them selectively react with imperfectly matched DNA. More recently, some therapeutic agents including DNA intercalating drugs and groove binders have been found to promote the in vivo repair system to recognize and repair the mismatch more effectively. The results have established a novel method for detection of mismatches. Development of new chemical reactions for detection of imperfectly matched DNA and mutations is a rapidly growing field and has attracted significant interest of scientists from both chemical and biological fields and it is the main focus of this review. (C) 2002 Elsevier Science (USA). All rights reserved.