Rapid visual detection of Escherichia coli and Vibrio cholerae Heat-labile enterotoxins by nitrocellulose enzyme-linked immunosorbent assay
Rapid visual detection of Escherichia coli and Vibrio cholerae Heat-labile enterotoxins by nitrocellulose enzyme-linked immunosorbent assay
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硝化纤维素酶联免疫吸附试验快速目视检测大肠杆菌和霍乱弧菌不耐热肠毒素
DOI：
10.1128/jcm.19.3.371-375.1984
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发表时间：
1984
影响因子：
9.4
通讯作者：
R. Stephan
中科院分区：
文献类型：
--
作者：
L. Beutin;L. Bode;T. Richter;G. Peltre;R. Stephan
对酶联免疫吸附试验进行了改进，建立了一种灵敏、快速、直观的检测大肠杆菌和霍乱弧菌不耐热肠毒素的方法。少量的细菌上清液结合在硝酸纤维素膜上，在硝酸纤维素酶联免疫吸附试验中用作吸附剂。这项测试是基于霍乱弧菌和大肠杆菌不耐热肠毒素之间的免疫学相似性。用硝酸纤维素酶联免疫吸附试验和Vero细胞生物试验对6株霍乱弧菌和48株大肠杆菌不耐热肠毒素进行了检测。对于某些菌株，硝酸纤维素酶联免疫吸附试验比Vero细胞试验更能敏感地检测不耐热肠毒素。对两种方法中不耐热肠毒素阳性的大肠杆菌H10407培养液进行终点滴定，得到了类似的结果。硝酸纤维素酶联免疫吸附试验检测纯化霍乱毒素的灵敏度为1 ng，与其他血清学方法相比具有较好的结果。
A modification of the enzyme-linked immunosorbent assay for a sensitive and rapid visual detection of heat-labile enterotoxins from Escherichia coli and Vibrio cholerae is described. Small amounts of bacterial supernatant fluids are bound to nitrocellulose filters which are used as sorbents in the nitrocellulose enzyme-linked immunosorbent assay. The test is based on the immunological similarity between V. cholerae and E. coli heat-labile enterotoxins. Six isolates of V. cholerae and 48 isolates of E. coli were examined for heat-labile enterotoxins by the nitrocellulose enzyme-linked immunosorbent assay and the Vero cell bioassay. With some strains, the nitrocellulose enzyme-linked immunosorbent assay was found to be more sensitive for detection of E. coli heat-labile enterotoxin than the Vero cell test. A similar result was obtained by endpoint titration of heat-labile enterotoxin-positive E. coli H10407 culture fluid in both assays. The sensitivity of the nitrocellulose enzyme-linked immunosorbent assay for the detection of purified cholera toxin was at a total level of 1 ng, which is a good result when compared with other serological assays.