Identification of Long Non-Coding RNAs and the Regulatory Network Responsive to Arbuscular Mycorrhizal Fungi Colonization in Maize Roots
Identification of Long Non-Coding RNAs and the Regulatory Network Responsive to Arbuscular Mycorrhizal Fungi Colonization in Maize Roots
复制标题
玉米根中丛枝菌根真菌定殖的长非编码 RNA 和调控网络的鉴定
DOI：
10.3390/ijms20184491
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发表时间：
2019-09-02
影响因子：
5.6
通讯作者：
Cheng, Beijiu
中科院分区：
文献类型：
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作者：
Han, Guomin;Cheng, Chen;Cheng, Beijiu
近年来，长链非编码RNA（lncRNA）已成为动植物许多生物学过程的重要调节因子。然而，据我们所知，迄今为止还没有关于植物对丛枝菌根（AM）真菌应答的lncRNA的研究报道。本研究利用链特异性RNA-Seq技术对AM真菌侵染的玉米根系进行分析，以鉴定AM真菌应答的lncRNA，并构建相关的调控网络。从接种Rhizophagus irregularis的玉米根中筛选出1837个差异表达的蛋白质编码基因。许多AM真菌响应基因与MtPt 4，STR，STR 2，MtFatM同源，并且丰富的途径如脂肪酸生物合成，对磷酸盐饥饿的响应和氮代谢与先前的研究一致。总共鉴定了5941个lncRNA，其中超过3000个是新的。其中，63个lncRNA差异表达。差异表达的lncRNAs（DELs）的靶基因主要与磷酸盐跨膜转运、细胞对钾离子饥饿的反应和脂质分解代谢过程有关。调控网络分析表明，DELs可能作为microRNA靶标参与调控植物与AM真菌之间的双向营养交换。本研究的结果可以拓宽我们对植物与AM真菌相互作用的认识。
Recently, long noncoding RNAs (lncRNAs) have emerged as vital regulators of many biological processes in animals and plants. However, to our knowledge no investigations on plant lncRNAs which respond to arbuscular mycorrhizal (AM) fungi have been reported thus far. In this study, maize roots colonized with AM fungus were analyzed by strand-specific RNA-Seq to identify AM fungi-responsive lncRNAs and construct an associated regulatory network. A total of 1837 differentially expressed protein coding genes (DEGs) were identified from maize roots with Rhizophagus irregularis inoculation. Many AM fungi-responsive genes were homologs to MtPt4, STR, STR2, MtFatM, and enriched pathways such as fatty acid biosynthesis, response to phosphate starvation, and nitrogen metabolism are consistent with previous studies. In total, 5941 lncRNAs were identified, of which more than 3000 were new. Of those, 63 lncRNAs were differentially expressed. The putative target genes of differentially expressed lncRNAs (DELs) were mainly related to phosphate ion transmembrane transport, cellular response to potassium ion starvation, and lipid catabolic processes. Regulatory network analysis showed that DELs might be involved in the regulation of bidirectional nutrient exchange between plant and AM fungi as mimicry of microRNA targets. The results of this study can broaden our knowledge on the interaction between plant and AM fungi.