Pharmacokinetics of plantamajoside and acteoside from Plantago asiatica in rats by liquid chromatography-mass spectrometry
Pharmacokinetics of plantamajoside and acteoside from Plantago asiatica in rats by liquid chromatography-mass spectrometry
复制标题
液相色谱-质谱法测定车前草苷和类叶升麻苷在大鼠体内的药代动力学
DOI：
10.1016/j.jpba.2013.11.014
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发表时间：
2014-02-15
影响因子：
3.4
通讯作者：
Deng, Yulin
中科院分区：
文献类型：
--
作者：
Li, Yujuan;Gan, Lin;Deng, Yulin
车前是一种药用和食用植物，富含多酚类化合物，如苯丙素苷、车前苷和毛蕊花苷。本研究旨在建立同时测定车前草苷和毛蕊花苷的新方法，并研究其在大鼠体内的药代动力学特性。采用液相色谱-串联质谱（LC-MS/MS）多反应监测（MRM）模式，对大鼠血浆中的2种药物进行定量分析。校准曲线在0.2-200 ng/ml范围内呈线性，两种分析物的相关系数均大于0.9983。车前草苷和毛蕊花苷的准确度分别为-4.2%~8.1%，相对误差为-3.8%~8.9%。两种分析物的精密度范围为2.7 - 10.2%相对标准偏差。药代动力学结果表明，车前草苷和毛蕊花苷在大鼠体内吸收迅速，血药浓度达峰时间分别为16.7 ± 2.8和13.3 ± 2.8min。车前草苷的消除常数为0.28 +/- 0.01 1 h（-1），类叶升麻苷的消除常数为0.47 +/- 0.03 1 h（-1）。所建立的方法和药动学数据为进一步研究积雪草酸的生物活性提供了基础。(C)2013爱思唯尔有限公司版权所有。
Plantago asiatica is a medicinal and dietary plant rich in polyphenolic compounds such as phenylpropanoid glycosides plantamajoside and acteoside. The aims of the present study were to develop a new and sensitive method for simultaneous determination of plantamajoside and acteoside and investigate their pharmacokinetic properties in rats. A sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method with multiple-reaction monitoring (MRM) mode was employed for quantification of two analytes in rat plasma. The calibration curve was linear over the range of 0.2-200 ng/ml with correlation coefficient greater than 0.9983 for both analytes. The accuracy of plantamajoside and acteoside were between -4.2% and 8.1%, -3.8% and 8.9% relative error, respectively. Precision for the two analytes ranged from 2.7 to 10.2% relative standard deviation. The pharmacokinetic results showed plantamajoside and acteoside were quickly absorbed in rat with the time to maximum plasma concentration 16.7 +/- 2.8 and 13.3 +/- 2.8 min, respectively. The elimination constants were 0.28 +/- 0.01 1 h(-1) for plantamajoside and 0.47 +/- 0.03 1 h(-1) for acteoside. The developed method and the pharmacokinetic data provide a basis for further studies on bioactivity of P. asiatica. (C) 2013 Elsevier B.V. All rights reserved.