Supramolecular host-guest pseudocomb conjugates composed of multiple star polycations tied tunably with a linear polycation backbone for gene transfection.
Supramolecular host-guest pseudocomb conjugates composed of multiple star polycations tied tunably with a linear polycation backbone for gene transfection.
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DOI：
10.1021/bc400115e
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发表时间：
2013-06
影响因子：
4.7
通讯作者：
Y. Hu;M. Chai;W. T. Yang;F. Xu
中科院分区：
文献类型：
--
作者：
Y. Hu;M. Chai;W. T. Yang;F. Xu
通过主客体相互作用，将低分子量β-环糊精（β-CD）核、乙醇胺功能化的聚甲基丙烯酸缩水甘油酯（PGEA）星星聚合物（CD-PGEAs）与金刚烷修饰的线性PGEA（l-PGEA-Ad）主链连接，合成了一系列新型超分子假梳聚阳离子（l-PGEA-Ad/CD-PGEAs）.在HepG 2和HEK 293细胞系中研究了假梳载体的DNA结合能力、细胞毒性和基因转染效率。pseudocomb l-PGEA-Ad/CD-PGEA具有较好的质粒DNA凝聚能力。同时，假梳状载体表现出较低的细胞毒性，与CD-PGEA和l-PGEA相似。此外，在不同PGEA氮/DNA磷酸摩尔比下，假梳状载体的基因转染效率远高于CD-PGEA和l-PGEA。这种假梳状基因载体的超分子制备可以提供一种灵活的方法，通过适当使用非共价相互作用来调节超分子聚合物的结构和功能。
A series of novel supramolecular pseudocomb polycations (l-PGEA-Ad/CD-PGEAs) were synthesized by tying multiple low-molecular-weight β-cyclodextrin (CD)-cored, ethanolamine-functionalized poly(glycidyl methacrylate) (PGEA) star polymers (CD-PGEAs) with an adamantine-modified linear PGEA (l-PGEA-Ad) backbone via the host-guest interaction. The pseudocomb carriers were studied in terms of their DNA binding capabilities, cytotoxicities, and gene transfection efficiencies in the HepG2 and HEK293 cell lines. The pseudocomb l-PGEA-Ad/CD-PGEAs exhibited better plasmid DNA-condensing abilities than their counterparts, CD-PGEA and l-PGEA. Meanwhile, the pseudocomb carriers displayed low cytotoxicity, similar to CD-PGEA and l-PGEA. Moreover, the gene transfection efficiencies of the pseudocomb carriers were much higher than those of CD-PGEA and l-PGEA at various PGEA nitrogen/DNA phosphate molar ratios. Such supramolecular preparation of pseudocomb gene carriers could provide a flexible approach for adjusting the structure and functionality of supramolecular polymers via the proper use of non-covalent interactions.