STRUCTURES OF CAPSULAR POLYSACCHARIDES OF NEISSERIA-MENINGITIDIS AS DETERMINED BY C-13-NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY
STRUCTURES OF CAPSULAR POLYSACCHARIDES OF NEISSERIA-MENINGITIDIS AS DETERMINED BY C-13-NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY
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DOI：
10.1093/infdis/136.supplement.s78
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发表时间：
1977-01-01
影响因子：
6.4
通讯作者：
SMITH, ICP
中科院分区：
文献类型：
--
作者：
JENNINGS, HJ;BHATTACHARJEE, AK;SMITH, ICP
本文报道了~(13)C-核磁共振波谱在脑膜炎奈瑟氏菌荚膜多糖抗原结构测定中的应用。对血清群A、B、C、W-135、X和Y的多糖谱进行了完整的指认，并且主要基于先前对各自多糖的单体单元所作的指认。该技术提供了多糖的所有结构方面的信息，包括组成、连接方式、O-乙酰取代基的位置、序列和构象。此外，核磁共振具有非破坏性、极快的特点，在细菌学研究中具有巨大的潜力。
The application of13C-nuclear magnetic resonance spectroscopy to the structural determination of the capsular polysaccharide antigens ofNeisseria meningitidisis described. Complete assignments of the spectra of the polysaccharides of serogroups A, B, C, W-135, X, and Y were made and were based mainly on previous assignments made for the monomer units of the respective polysaccharides. This technique provides information on all structural aspects of the polysaccharides including composition, mode of linkage, location of O-acetyl substituents, seque{lce, and conformation. In addition, nuclear magnetic resonance is nondestructive, extremely rapid, and has enormous potential in bacteriological research.