A cotton Raf-like MAP3K gene, GhMAP3K40, mediates reduced tolerance to biotic and abiotic stress in Nicotiana benthamiana by negatively regulating growth and development
A cotton Raf-like MAP3K gene, GhMAP3K40, mediates reduced tolerance to biotic and abiotic stress in Nicotiana benthamiana by negatively regulating growth and development
复制标题
棉花 Raf 样 MAP3K 基因 GhMAP3K40 通过负向调节生长和发育来介导本塞姆氏烟草对生物和非生物胁迫的耐受性降低。
DOI：
10.1016/j.plantsci.2015.08.012
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发表时间：
2015-11-01
期刊：
影响因子：
5.2
通讯作者：
Guo, Xingqi
中科院分区：
文献类型：
--
作者：
Chen, Xiaobo;Wang, Ji;Guo, Xingqi
丝裂原活化蛋白激酶(MAPK)级联通路在植物体内介导多种反应。作为MAP3K5的顶端成分，MAP3K5值得更多的关注；然而，人们对MAP3Ks的作用知之甚少，特别是在棉花这一世界性经济作物中的作用。在本研究中，我们分离到一个编码类似Raf的MAP3K的基因GhMAP3K40。GhMAP3K40在应激和多种信号分子诱导下表达。在萌发期，高表达GhMAP3K40的植株对干旱和盐胁迫的耐受性增强。然而，在苗期，转基因植株在干旱、病原体暴露和氧化胁迫后遭受了更严重的伤害。在转基因涂料中，防御相关基因和抗氧化系统被激活，这表明GhMAP3K40对防御反应具有积极的调节作用。转基因植株阻止病原菌入侵的能力较差，这是由于叶片细胞结构的缺陷造成的。与转基因植株相比，对照植株的根系较强。这些结果表明GhMAP3K40在生长发育过程中起着负性作用，GhMAP3K40可能是通过下调木质素的生物合成而导致缺陷的。总体而言，这些结果表明GhMAP3K40可能正向调节防御反应，但通过负向调节生长发育而降低对生物和非生物胁迫的耐受性。(C)2015爱思唯尔爱尔兰有限公司。保留所有权利。
Mitogen-activated protein kinase (MAPK) cascades mediate various responses in plants. As the top component, MAP3K5 deserve more attention; however, little is known about the role of MAP3Ks, especially in cotton, a worldwide economic crop. In this study, a gene encoding a putative Raf-like MAP3K, GhMAP3K40, was isolated. GhMAP3K40 expression was induced by stress and multiple signal molecules. The plants overexpressing GhMAP3K40 had an enhanced tolerance to drought and salt stress at the germination stage. However, at the seedling stage, the transgenic plants suffered more severe damage after drought, exposure to pathogens and oxidative stress. The defence-related genes and the antioxidant system were activated in transgenic paints, suggesting that GhMAP3K40 positively regulate the defence response. The transgenic plants were less able to prevent pathogenic invasion, which was due to defects in the cell structure of the leaves. The root system of the control plants were stronger compared with the transgenic plants. These results indicated a negative role of GhMAP3K40 in growth and development and GhMAP3K40 possibly caused the defects by down-regulating the lignin biosynthesis. Overall, these results suggest that GhMAP3K40 may positively regulate defence response but cause reduced tolerance to biotic and abiotic stress by negatively regulating growth and development. (C) 2015 Elsevier Ireland Ltd. All rights reserved.