Protection against anthrax toxin by vaccination with a DNA plasmid encoding anthrax protective antigen
Protection against anthrax toxin by vaccination with a DNA plasmid encoding anthrax protective antigen
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DOI：
10.1016/s0264-410x(98)00210-2
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发表时间：
1999-01-28
期刊：
影响因子：
5.5
通讯作者：
Klinman, DM
中科院分区：
文献类型：
--
作者：
Gu, ML;Leppla, SH;Klinman, DM
A DNA vaccine encoding the immunogenic and biologically active portion of anthrax protective antigen (PA) was constructed. Spleen cells from BALB/c mice immunized intramuscularly with this vaccine were stimulated to secrete IFN gamma and IL-4 when exposed to PA in vitro. Immunized mice also mounted a humoral immune response dominated by IgG1 anti-PA antibody production, the subclass previously shown to confer protection against anthrax toxin. A 1:100 dilution of serum from these animals protected cells in vitro against cytotoxic concentrations of PA. Moreover, 7/8 mice immunized three times with the PA DNA vaccine were protected against lethal challenge with a combination of anthrax protective antigen plus lethal factor. Published by Elsevier Science Ltd.
