Idiotypic determinants on human T cells and modulation of human T cell responses by anti-idiotypic antibodies.
Idiotypic determinants on human T cells and modulation of human T cell responses by anti-idiotypic antibodies.
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人类 T 细胞的独特型决定因素以及抗独特型抗体对人类 T 细胞反应的调节。
DOI：
10.4049/jimmunol.133.4.1846
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发表时间：
1984
影响因子：
4.4
通讯作者：
R. Geha
中科院分区：
文献类型：
--
作者：
R. Geha
在三个受试者中研究了独特型抗独特型相互作用在人T细胞对破伤风类毒素（TT）抗原反应的调节中的作用。兔抗独特型（anti-Id）抗血清是在TT加强免疫后7 ~ 10天获得的IgG （Fab’）2抗TT血清。F（ab’）2兔抗兔IgG片段与荧光素偶联的山羊抗兔IgG结合，采用间接免疫荧光法测定T细胞富集制剂中id阳性细胞的频率。在被研究的三个对象中，这一频率分别为万分之24、29和38。通过山羊抗人Ig （GAHIG）盖顶实验和罗丹明共轭GAHIG双染色实验，排除了污染B细胞对荧光染色的显著贡献。用eb病毒（EBV）转化的IgG （Fab’）2抗tt供者的B细胞系吸收抗- id抗血清，而不用来自无亲缘关系供者的EBV-B细胞系吸收抗- id抗血清，消除了它们与T细胞的反应性。兔抗- IgG对T细胞增殖的影响最小（2 - 3倍），对TT抗原应答的T细胞增殖无影响。将T细胞与兔抗id IgG （Fab’）2预孵育48小时，而不是与免疫前兔IgG （Fab’）2预孵育48小时，结果产生抗原特异性抑制细胞，抑制T细胞对TT的增殖，但对白喉类毒素（DT）没有反应。这些细胞对TT抗原的体外刺激也能抑制抗TT IgG的合成，但对DT抗原的合成无抑制作用。T细胞在兔抗id IgG （Fab’）2包被板上的吸附增强了T细胞对TT抗原的增殖反应，而对DT抗原的增殖反应没有增强，并且增强了T细胞体外合成抗TT抗体IgG的辅助活性，而不是抗DT抗体IgG的辅助活性。这些结果表明，独特型-抗独特型相互作用在人T细胞对抗原的反应中起作用。
The role of idiotypic anti-idiotypic interactions in the regulation of the human T cell response to tetanus toxoid (TT) antigen was examined in three subjects. Rabbit anti-idiotypic (anti-Id) antisera were raised against IgG (Fab')2 anti-TT obtained 7 to 10 days after booster immunization with TT. F(ab')2 fragments of rabbit-anti-Id IgG were used in conjunction with fluorescein-conjugated goat anti-rabbit Ig in an indirect immunofluorescence assay to determine the frequency of Id-positive cells in T cell-enriched preparations. This frequency was 24, 29, and 38 per 10,000, respectively, in the three subjects studied. Significant contribution of contaminating B cell to fluorescence-staining was ruled out by capping experiments using goat anti-human Ig (GAHIG) and by double staining experiments using rhodamine-conjugated GAHIG. Absorption of anti-Id antisera with Epstein Barr virus (EBV)-transformed B cell lines from the IgG (Fab')2 anti-TT donor, but not with EBV-B cell lines from unrelated donors, removed their reactivity with the T cells. Rabbit anti-Id IgG caused minimal proliferation (two-threefold) of T cells and had no effect on T cell proliferation in response to TT antigen when added to the cultures. Preincubation of T cells for 48 hr with rabbit anti-Id IgG (Fab')2, but not with preimmune rabbit IgG (Fab')2, resulted in the generation of antigen-specific suppressor cells that inhibited T cell proliferation in response to TT, but not in response to diphtheria toxoid (DT). These cells also inhibited the synthesis of IgG anti-TT in response to in vitro stimulation with TT antigen, but not the synthesis of IgG anti-DT in response to DT antigen. Adsorption of T cells over plates coated with rabbit anti-Id IgG (Fab')2 enhanced the proliferative response of the T cells to TT, but not to DT antigen, and enhanced the helper activity of the T cells for the in vitro synthesis of IgG anti-TT but not of IgG anti-DT antibodies. These results suggest that idiotypic-anti-idiotypic interactions play a role in the human T cell response to antigen.