Gonadal transcriptomic analysis and differentially expressed genes between the testes and ovaries in Trachinotus ovatus
Gonadal transcriptomic analysis and differentially expressed genes between the testes and ovaries in Trachinotus ovatus
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卵形鲳鲳性腺转录组分析及睾丸和卵巢差异表达基因
DOI：
10.1016/j.aaf.2020.09.007
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发表时间：
2022-01-01
影响因子：
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通讯作者：
Peng, Jinxia
中科院分区：
文献类型：
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作者：
He, Pingping;Zhu, Peng;Peng, Jinxia
卵形鲳鲹是我国重要的水产养殖品种。雄性和雌性鱼之间没有明显的形态差异，即使在成熟期，也促使人们对这种鱼的性别相关特征进行研究。为了检测性别决定和性腺发育相关基因，我们对T。卵形的从12个文库中共获得了345，972，132个高质量的干净读段。此外，通过将干净读段定位到T.卵形参考基因组。在I期卵巢和睾丸之间共鉴定了8，237个差异表达基因（DEG），包括3，235个睾丸上调基因和5，002个卵巢上调基因。此外，在III期卵巢和睾丸文库之间获得了13，448个DEG，包括7，576个睾丸上调基因和5，872个卵巢上调基因。DEG包括一些性别决定基因，如sry，dmrt 1和amh。卵巢和睾丸文库之间的DEG显著富集有参与性腺发育、性别决定和性腺功能的KEGG途径。然后，选择10个DEG（7个睾丸上调和3个卵巢上调的unigenes）进行定量实时PCR分析。四个基因（锌结合蛋白A33样，前阿黑皮素样，亮氨酸丰富的重复和跨膜结构域包含蛋白2样，叉头boxC1）显示了一个特定的睾丸表达模式。性腺、睾丸和卵巢表达的DEG为进一步研究T.卵形的
The Trachinotus ovatus is a popular aquaculture species in China. There are no obvious morphological differences between male and female fish, even during maturity, prompting research studies on sex-related features of this fish. To examine sex determination- and gonadal development-related genes, we conducted transcriptome analysis of the ovaries and testes of T. ovatus. A total of 345,972,132 high-quality clean reads were obtained from 12 libraries. In addition, 28,137 gonad-expressed unigenes were obtained by mapping the clean reads to the T. ovatus reference genome. A total of 8,237 differentially expressed genes (DEGs) were identified between stage I ovaries and testes, including 3,235 testicular upregulated and 5,002 ovarian upregulated genes. Furthermore, 13,448 DEGs were obtained between stage III ovarian and testicular libraries, including 7,576 testicular upregulated and 5,872 ovarian upregulated genes. The DEGs included some sex-determining genes such as sry, dmrt1, and amh. DEGs between ovarian and testicular libraries were significantly enriched with KEGG pathways that are involved in gonadal development, sex determination, and gonadal function. Then, 10 DEGs (seven testicular upregulated and three ovarian upregulated unigenes) were selected for quantitative real-time PCR analysis. Four genes (zinc-binding protein A33-like, pro-opiomelanocortin-like, leucine-rich repeat and transmembrane domain-containing protein 2-like, and forkhead boxC1) showed a specific testicular expression pattern. The gonadally expressed as well as testicular and ovarian DEGs provide useful information for further research on the reproductive biology of T. ovatus.