Combining Chromatin Immunoprecipitation and Oligonucleotide Tiling Arrays (ChIP-Chip) for Functional Genomic Studies
Combining Chromatin Immunoprecipitation and Oligonucleotide Tiling Arrays (ChIP-Chip) for Functional Genomic Studies
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DOI：
10.1007/978-1-60327-192-9_11
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Brown, Myles
中科院分区：
文献类型：
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作者：
Eeckhoute, Jerome;Lupien, Mathieu;Brown, Myles
系统生物学的核心是全基因组技术和高通量实验方法。人类基因组测序的完成，以及其他一些高等真核生物的测序，现在允许第一次映射的所有顺式调控区的基因，以及核小体的位置和修饰的细节。实现这一目标的一种方法涉及染色质免疫沉淀结合DNA寡核苷酸平铺阵列（ChIP芯片）。这允许通过在覆盖整个基因组或感兴趣的特定区域的平铺阵列上杂交来鉴定由给定因子、其顺式组结合的基因组区域或含有给定表观基因组修饰的基因组区域。该技术提供了对任何DNA相关因子或表观基因组标记的潜在生物学功能的所有公正评估。通过将ChIP芯片数据与互补的全基因组方法（包括表达谱、CGH和SNP阵列）整合，转录调控和染色质结构的新范式正在出现。
Central to systems biology are genome-wide technologies and high-throughput experimental approaches. Completion of the sequencing of the human genome as well as those of a number of other higher eukaryotes now allows for the first time the mapping of all of the cis-regulatory regions of genes as well as the details of nucleosome position and modification. One approach to achieving this goal involves chromatin immunoprecipitation combined with DNA oligonucleotide tiling arrays (ChIP-chip). This allows for the identification of genomic regions bound by a given factor, its cistrome, or harboring a given epigenomic modification through hybridization on tiling arrays covering the entire genome or specific regions of interest. This technology offers all unbiased assessment of the potential biological function of any DNA associated factor or epigenomic mark. Through integration of ChIP-chip data with complementary genome-wide approaches including expression profiling, CGH and SNP arrays, novel paradigms of transcriptional regulation and chromatin structure are emerging.