Determination of novel Borrelia genospecies in Swedish Ixodes ricinus ticks
Determination of novel Borrelia genospecies in Swedish Ixodes ricinus ticks
复制标题
DOI：
10.1128/jcm.40.9.3308-3312.2002
复制
发表时间：
2002-09-01
影响因子：
9.4
通讯作者：
Berglund, J
中科院分区：
文献类型：
--
作者：
Fraenkel, CJ;Garpmo, U;Berglund, J
在瑞典南部和东海岸 15 个不同地点收集了总共 301 只成年蓖麻硬蜱，以确定疏螺旋体基因种多样性。使用伯氏疏螺旋体特异性引物进行巢式 PCR 检测，发现 32 只蜱 (11%) 呈阳性。物种测定基于 16S rRNA 基因和鞭毛蛋白基因的部分测序。发现了五种不同的疏螺旋体属物种。两种蜱中发现的疏螺旋体 DNA 的核苷酸序列与其他蜱中发现的疏螺旋体 DNA 的核苷酸序列存在很大差异，分析表明它们与回归热疏螺旋体物种宫本疏螺旋体 (Borrelia miyamotoi) 密切相关。这是在蓖麻属和欧洲首次报道类似 Miyanzotoi 的疏螺旋体。此外，两只蜱虫 (6%) 的疏螺旋体 DNA 聚集在瓦莱疏螺旋体复合体内。瑞典此前未曾报道过瓦莱 B. valaisiana。在 14 只蜱虫 (44%) 中发现了 B. afzelii DNA，在 10 只蜱虫 (31%) 中发现了 B. garinii DNA。在四只蜱 (13%) 中发现了严格伯氏疏螺旋体 DNA。我们得出的结论是，在欧洲其他地方发现的所有已知的人类致病物种（B. garinii、B. afzelii 和 B. burgdorferi sensu stricto）和 B. valaisiana 也存在于瑞典寻求寄主的蜱种群中，并且类似宫本疏螺旋体的疏螺旋体物种似乎存在于欧洲的蓖麻蜱中。
A total of 301 adult questing Ixodes ricinus ticks were collected at 15 different locations along the South and east coasts of Sweden to determine the Borrelia genospecies diversity. Thirty-two ticks (11%) were found to be positive by nested PCR with Borrelia burgdorferi sensu lato-specific primers. Species determination was based on partial sequencing of the 16S rRNA gene and the flagellin gene. Five different Borrelia species were found. The nucleotide sequence of the Borrelia DNA found in two ticks differed extensively from the nucleotide sequences of the Borrelia DNA found in the other ticks, and analysis revealed that they were closely related to the relapsing fever borrelia species Borrelia miyamotoi. This is the first report of a B. miyanzotoi-like borrelia in L ricinus and in Europe. Moreover, the Borrelia DNA of two ticks (6%) clustered within the B. valaisiana complex. B. valaisiana has not previously been reported in Sweden. B. afzelii DNA was found in 14 ticks (44%), and B. garinii DNA was found in 10 ticks (31%). B. burgdorferi sensu stricto DNA was found in four ticks (13%). We conclude that all of the known human-pathogenic species (B. garinii, B. afzelii, and B. burgdorferi sensu stricto) and B. valaisiana found elsewhere in Europe are also present in the Swedish host-seeking tick population and that a B. miyamotoi-like Borrelia species seems to be present in L ricinus ticks in Europe.