AtWRKY22 promotes susceptibility to aphids and modulates salicylic acid and jasmonic acid signalling.
AtWRKY22 promotes susceptibility to aphids and modulates salicylic acid and jasmonic acid signalling.
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DOI：
10.1093/jxb/erw159
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发表时间：
2016-05
影响因子：
6.9
通讯作者：
Jongsma MA
中科院分区：
文献类型：
--
作者：
Kloth KJ;Wiegers GL;Busscher-Lange J;van Haarst JC;Kruijer W;Bouwmeester HJ;Dicke M;Jongsma MA
转录因子WRKY 22通过抑制水杨酸信号增加拟南芥对蚜虫的易感性。蚜虫在其宿主植物中诱导许多转录扰动，但负责的信号级联和对植物抗性的影响在很大程度上是未知的。通过拟南芥全基因组关联（GWA）定位研究，我们确定WRKY 22作为一个候选基因与绿色桃蚜，桃蚜的取食行为。已知转录因子WRKY 22参与病原体触发的免疫，并且WRKY 22基因表达已被证明由蚜虫诱导。对敲除突变体和过表达品系上蚜虫种群发育和取食行为的评估表明，WRKY 22增加了对M. persicae通过叶肉定位机制。对蚜虫侵染的wrky 22基因敲除植物的mRNA测序分析显示，参与水杨酸（SA）信号传导的基因上调，参与植物生长和细胞壁松动的基因下调。此外，机械刺激敲除植物的夹笼上调茉莉酸（JA）响应基因，导致大量的负JA-SA串扰。基于这一点和以前的研究，WRKY 22被认为是调节SA和JA途径之间的相互作用，以响应广泛的生物和非生物刺激。它的诱导蚜虫和它的作用，抑制SA和JA信号使WRKY 22蚜虫操纵宿主植物防御的潜在目标。
The transcription factor WRKY22 increases susceptibility to aphids in Arabidopsis via the suppression of salicylic acid signalling. Aphids induce many transcriptional perturbations in their host plants, but the signalling cascades responsible and the effects on plant resistance are largely unknown. Through a genome-wide association (GWA) mapping study in Arabidopsis thaliana, we identified WRKY22 as a candidate gene associated with feeding behaviour of the green peach aphid, Myzus persicae. The transcription factor WRKY22 is known to be involved in pathogen-triggered immunity, and WRKY22 gene expression has been shown to be induced by aphids. Assessment of aphid population development and feeding behaviour on knockout mutants and overexpression lines showed that WRKY22 increases susceptibility to M. persicae via a mesophyll-located mechanism. mRNA sequencing analysis of aphid-infested wrky22 knockout plants revealed the up-regulation of genes involved in salicylic acid (SA) signalling and down-regulation of genes involved in plant growth and cell-wall loosening. In addition, mechanostimulation of knockout plants by clip cages up-regulated jasmonic acid (JA)-responsive genes, resulting in substantial negative JA–SA crosstalk. Based on this and previous studies, WRKY22 is considered to modulate the interplay between the SA and JA pathways in response to a wide range of biotic and abiotic stimuli. Its induction by aphids and its role in suppressing SA and JA signalling make WRKY22 a potential target for aphids to manipulate host plant defences.