Genetic characterization of Italian honeybees, Apis mellifera ligustica, based on microsatellite DNA polymorphisms
Genetic characterization of Italian honeybees, Apis mellifera ligustica, based on microsatellite DNA polymorphisms
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DOI：
10.1051/apido:2006073
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发表时间：
2007-03-01
期刊：
影响因子：
2.4
通讯作者：
Moritz, Robin F. A.
中科院分区：
文献类型：
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作者：
Dall'Olio, Raffaele;Marino, Alberto;Moritz, Robin F. A.
用8个多态性微卫星位点对意大利蜜蜂（Apis mellifera ligustica）在意大利半岛和撒丁岛的遗传变异进行了筛选。对意大利蜜蜂、意大利蜜蜂肉种和来自Buckfast育种系的样品进行基因分型以进行比较。低Fis和Fst值表明，A. M. ligustica种群，虽然检测到的等位基因数量和杂合性高。系统发育和个体分析证实了A. M. ligustica已经成为一个大的种群，可能是由于密集的养蜂做法，如迁移养蜂和大规模的商业蜂王交易。由于外来等位基因渗入到地方性的天然和商业A。M.通过微卫星分析，可以对川芎种群进行检测和监测，为今后当地生物多样性保护和其他控制育种计划提供参考数据。
The genetic variability of Apis mellifera ligustica was screened throughout the Italian peninsula and Sardinia with eight polymorphic microsatellite loci. Samples of Apis mellifera mellifera, Apis mellifera carnica and from the Buckfast breeding line were genotyped for comparison. Low Fis and Fst values suggest the absence of genetic structure within and among A. m. ligustica populations, although the high number of alleles detected and heterozygosity. Phylogenetic and individual analyses confirmed that A. m. ligustica has come to resemble one large population, probably as a result of intensive beekeeping practices such as migratory beekeeping and large-scale commercial queen trading. Since the introgression of foreign alleles into both endemic natural and commercial A. m. ligustica populations, can be detected and monitored by microsatellite analysis, the results provide a reference data set for future local biodiversity conservation and other controlled breeding programs.