Isolation of SPO12-1 and SPO13-1 from a natural variant of yeast that undergoes a single meiotic division.
Isolation of SPO12-1 and SPO13-1 from a natural variant of yeast that undergoes a single meiotic division.
复制标题
从经历单次减数分裂的酵母天然变体中分离 SPO12-1 和 SPO13-1。
DOI：
10.1093/genetics/96.3.567
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发表时间：
1980
期刊：
影响因子：
3.3
通讯作者：
Esposito,RE
中科院分区：
文献类型：
--
作者：
Klapholz,S;Esposito,RE
ATCC 4117是一株S.在孢子形成过程中经历一次核分裂产生含有两个二倍体子囊孢子的子囊（Grewal和米勒1972）。从这些孢子衍生的所有克隆都具有孢子形成能力，并且与亲本菌株一样，形成双孢子子囊。在本文中，我们描述了ATCC 4117的遗传分析。在ATCC 4117二倍体与α/α或α/α二倍体营养细胞的四倍体杂种中，双孢子子囊的产生是隐性的。从这些四倍体中，我们分离出两个隐性等位基因，命名为spo 12 - 1和spo 13 -1，每一个单独都会产生带有两个二倍体或近二倍体孢子的子囊。这些等位基因是不连锁和分离的单核基因。spo 12 - 1是约22厘米，从它的着丝粒，spo 13 - 1已定位到1厘米内的arg 4对染色体VIII。该分析还揭示了ATCC 4117携带与HO等位或紧密连锁的二倍体化基因，该基因是减少每孢子的单倍体孢子数量的修饰剂，并且等位基因影响孢子形成的总水平。
ATCC4117 is a strain ofS. cerevisiaethat undergoes a single nuclear division during sporulation to produce asci containing two diploid ascospores (Grewal and Miller 1972). All clones derived from these spores are sporulation-capable and, like the parental strain, form two-spored asci. In this paper, we describe the genetic analysis of ATCC4117. In tetraploid hybrids of vegetative cells of the ATCC4117 diploid anda/aor α/α diploids, the production of two-spored asci is recessive. From these tetraploids, we have isolated two recessive alleles, designatedspo12–1andspo13–1, each of which alone results in the production of asci with two diploid or near-diploid spores. These alleles are unlinked and segregate as single nuclear genes.spo12–1is approximately 22 cM from its centromere;spo13–1has been localized to within 1 cM ofarg4on chromosomeVIII. This analysis also revealed that ATCC4117 carries a diploidization gene allelic to or closely linked toHO, modifiers that reduce the number of haploid spores per ascus and alleles affecting the total level of sporulation.