A functional biphasic biomaterial homing mesenchymal stem cells for in vivo cartilage regeneration
A functional biphasic biomaterial homing mesenchymal stem cells for in vivo cartilage regeneration
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DOI：
10.1016/j.biomaterials.2014.08.020
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发表时间：
2014-12-01
期刊：
影响因子：
14
通讯作者：
Ao, Yingfang
中科院分区：
文献类型：
--
作者：
Huang, Hongjie;Zhang, Xin;Ao, Yingfang
由于关节负重、滑膜运动和内源性修复细胞缺乏等原因，创伤后软骨再生仍然是临床医生和研究人员面临的巨大挑战。为了克服这些局限性，我们使用双相支架平台和骨源性间充质干细胞（BMSCs）特异性亲和肽构建了一种功能性生物材料。双相支架平台在壳聚糖的溶胶-凝胶转变中均匀地保留更多的细胞，并提供足够的固体基质强度。该双相支架平台用靶向感兴趣的细胞来源BMSC的亲和肽功能化。缀合肽的存在赋予该系统在体外和体内朝向BMSC特异性归巢的生物学功能。该功能性生物材料可以在体外培养期间刺激干细胞增殖和软骨形成分化。体内植入后6个月，与常规手术或对照支架相比，功能性生物材料诱导了上级软骨修复，无并发症，如组织学观察、磁共振成像和生物力学特性所示。除了软骨修复之外，这种功能性双相支架可以通过归巢内源性细胞来刺激组织再生，为一步组织工程策略提供生物材料框架。(C)2014爱思唯尔有限公司版权所有。
Cartilage regeneration after trauma is still a great challenge for clinicians and researchers due to many reasons, such as joint load-bearing, synovial movement and the paucity of endogenous repair cells. To overcome these limitations, we constructed a functional biomaterial using a biphasic scaffold platform and a bone-derived mesenchymal stem cells (BMSCs)-specific affinity peptide. The biphasic scaffold platform retains more cells homogeneously within the sol-gel transition of chitosan and provides sufficient solid matrix strength. This biphasic scaffold platform is functionalized with an affinity peptide targeting a cell source of interest, BMSCs. The presence of conjugated peptide gives this system a biological functionality towards BMSC-specific homing both in vitro and in vivo. The functional biomaterial can stimulate stem cell proliferation and chondrogenic differentiation during in vitro culture. Six months after in vivo implantation, compared with routine surgery or control scaffolds, the functional biomaterials induced superior cartilage repair without complications, as indicated by histological observations, magnetic resonance imaging and biomechanical properties. Beyond cartilage repair, this functional biphasic scaffold may provide a biomaterial framework for one-step tissue engineering strategy by homing endogenous cells to stimulate tissue regeneration. (C) 2014 Elsevier Ltd. All rights reserved.