Allergenicity of mare's milk in children with cow's milk allergy
Allergenicity of mare's milk in children with cow's milk allergy
复制标题
DOI：
10.1067/mai.2000.106377
复制
发表时间：
2000-05-01
影响因子：
14.2
通讯作者：
Orlandi, M
中科院分区：
文献类型：
--
作者：
Businco, L;Giampietro, PG;Orlandi, M
背景：牛奶过敏是婴幼儿常见病。如果婴儿不是母乳喂养的，牛奶配方的替代品是必要的。目的：本研究的目的是调查，在体外和体内，马奶的变应原性在一个人口的选定儿童与严重的IgE介导的牛奶过敏。方法：25名儿童（17名男性和8名女性），年龄19至72个月（中位年龄34个月）与IgE介导的con的牛奶过敏被选为这项研究。所有儿童都接受了牛奶和马奶的皮肤点刺试验和新鲜牛奶、新鲜马奶的双盲安慰剂对照口服食物挑战（DBP-COFC），以及作为安慰剂的大豆配方（Isomil，Abbott. Campoverde，意大利）。我们进行免疫印迹的牛奶和马奶过敏children.Results：所有的孩子都表现出强烈的阳性皮肤试验反应牛奶（4+），2名儿童有阳性皮肤试验反应马奶（2+）。所有儿童对牛奶的DBP-COFC均呈阳性;一名儿童对马奶的DBP-COFC呈阳性。没有儿童对安慰剂（lsomil）有反应。在牛奶中，一些蛋白质能够强烈地与人IgE反应，当血清与马奶测试，对应于相同的蛋白质的条带被识别的较低的百分比serum.Conclusion：这些数据表明，马奶可以被视为一个很好的替代牛奶中的大多数儿童严重的IgE介导的牛奶过敏。然而，谨慎的做法是通过监督滴定口服激发试验确认其耐受性。
Background: Cow's milk allergy is a common disease of infancy and early childhood. If the baby is not breast-fed, a substitute for cow's milk formula is necessary.Objective: The aim of this study was to investigate, in vitro and in vivo, the allergenicity of mare's milk in a population of selected children with severe IgE-mediated cow's milk allergy.Methods: Twenty-five children (17 male and 8 female) aged 19 to 72 months (median age 34 months) with IgE-mediated con's milk allergy were selected for this study. All the children underwent skin prick tests with cow's milk and mare's milk and double-blind placebo-controlled oral food challenge (DBP-COFC) with fresh cow's milk, fresh mare's milk, and, as placebo, a soy formula (Isomil, Abbott. Campoverde, Italy). We performed immunoblotting of cow's and mare's milk developed with IgE from allergic children.Results: All the children showed strong positive skin test responses to cow's milk (4+); 2 children had positive skin test responses to mare's milk (2+). All children had positive DBP-COFCs to cow's milk; one child had a positive DBP-COFC to mare's milk. No children reacted to the placebo (lsomil). In the cow's milk, some proteins are able to strongly react with human IgE; when the sera are tested with mare's milk, the bands corresponding to the same proteins are recognized by a lower percentage of sera.Conclusion: These data suggest that mare's milk can be regarded as a good substitute of cow's milk in most children with severe IgE-mediated cow's milk allergy. It would be prudent, however, to confirm its tolerability by a supervised titrated oral challenge test.