Leaflet initiation is temporally and spatially separated in simple and complex tomato (Solanum lycopersicum) leaf mutants: a developmental analysis
Leaflet initiation is temporally and spatially separated in simple and complex tomato (Solanum lycopersicum) leaf mutants: a developmental analysis
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DOI：
10.1139/b10-051
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发表时间：
2010-08-01
期刊：
影响因子：
1.1
通讯作者：
Sinha, Neelima R.
中科院分区：
文献类型：
--
作者：
Kang, Julie;Sinha, Neelima R.
复叶的形成需要多种因素的参与，包括KNOX 1基因的表达。为了进一步表征简单和复杂的番茄叶突变体，我们分析了它们的形态和发展评估：叶表型，初级叶形态发生，表达的I类KNOX基因LeT 6，和分生组织活动的边缘blastozone。分析具有裂片和（或）羽状（减少/增加）改变的突变体。在叶状减少的突变体中，初级小叶起始延迟。与此相反，小叶起始是先进的或类似的野生型突变体深叶。叶与增加羽状沿着轴需要一个长期的发展计划，以形成其最终的叶形态。使用形态分析，我们表明，叶的复杂性可以量化。LeT 6的表达模式与组织学分析的分生组织活动的边缘blastozone，这表明，LeT 6可能发挥作用，通过一些未知的机制，调节分生组织的能力，不仅在边缘blastozone调节小叶裂片，但沿着整个长度的叶调节羽状复叶。
Formation of a compound leaf requires the involvement of multiple factors, including KNOX1 gene expression. To further characterize simple and complex tomato leaf mutants, we analyzed their morphology and development by assessing: leaf phenotypes, primary leaf morphogenesis, expression of the class I KNOX gene LeT6, and meristematic activity of the marginal blastozone. Mutants with alterations in lobing and (or) pinnation (decrease/increase) were analyzed. Primary leaflet initiation is delayed in mutants with decreased lobing. In contrast, leaflet initiation is advanced or similar to the wild type in mutants with deep lobes. Leaves with increased pinnation along the rachis require a protracted developmental program to form their final leaf morphology. Using a morphometric analysis, we show that leaf complexity can be quantified. The expression pattern of LeT6 correlates with histological analysis of meristematic activity of the marginal blastozone, suggesting that LeT6 may play a role, through some unknown mechanism, to regulate meristematic competence, not only in the marginal blastozone to regulate leaflet lobing, but along the entire length of the leaf to regulate pinnation in compound leaves.