Cell population dynamics modulate the rates of tissue growth processes
Cell population dynamics modulate the rates of tissue growth processes
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DOI：
10.1529/biophysj.105.063701
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发表时间：
2006-02-01
影响因子：
3.4
通讯作者：
Zygourakis, K
中科院分区：
文献类型：
--
作者：
Cheng, G;Youssef, BB;Zygourakis, K
介绍了一种描述三维支架中组织生长动态过程的离散模型的开发和测试。该模型考虑了在计算网格上执行持久随机行走、碰撞和扩散直到达到汇合点的细胞群体。为了分离种群动态对组织生长的影响，该模型假设营养物质和生长因子的浓度在空间和时间上保持不变。模拟从种子细胞在支架中均匀随机分布开始，或者从设计用于模拟伤口愈合的迁移和细胞增殖阶段的初始条件开始。均匀播种的模拟表明，细胞迁移通过抵消接触抑制的不利影响来促进组织生长。然而，当迁移速度较快时，这种有益影响会减弱并完全消失。相比之下，“创伤”种植模式的模拟显示，随着细胞迁移速度的增加，组织再生率不断提高。我们得出结论，细胞运动参数和种子细胞的空间分布可以对这一过程的动力学产生深远的影响，从而影响组织生长的模式和速度。这些结果可以指导仿生模型有效性检验的实验设计。刺激组织生长的阳离子。
The development and testing of a discrete model describing the dynamic process of tissue growth in three-dimensional scaffolds is presented. The model considers populations of cells that execute persistent random walks on the computational grid, collide, and proliferate until they reach confluence. To isolate the effect of population dynamics on tissue growth, the model assumes that nutrient and growth factor concentrations remain constant in space and time. Simulations start either by distributing the seed cells uniformly and randomly throughout the scaffold, or from an initial condition designed to simulate the migration and cell proliferation phase of wound healing. Simulations with uniform seeding show that cell migration enhances tissue growth by counterbalancing the adverse effects of contact inhibition. This beneficial effect, however, diminishes and disappears completely for large migration speeds. By contrast, simulations with the "wound" seeding mode show a continual enhancement of tissue regeneration rates with increasing cell migration speeds. We conclude that cell locomotory parameters and the spatial distribution of seed cells can have profound effects on the dynamics of the process and, consequently, on the pattern and rates of tissue growth. These results can guide the design of experiments for testing the effectiveness of biomimetic modi. cations for stimulating tissue growth.