Co-Expression of Chicken IL-2 and IL-7 Enhances the Immunogenicity and Protective Efficacy of a VP2-Expressing DNA Vaccine against IBDV in Chickens
Co-Expression of Chicken IL-2 and IL-7 Enhances the Immunogenicity and Protective Efficacy of a VP2-Expressing DNA Vaccine against IBDV in Chickens
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DOI：
10.3390/v11050476
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发表时间：
2019-05-01
期刊：
影响因子：
4.7
通讯作者：
Zhong, Fei
中科院分区：
文献类型：
--
作者：
Huo, Shanshan;Zhang, Jianlou;Zhong, Fei
鸡传染性法氏囊病（IBD）在世界范围内仍未得到完全控制。IBD病毒（IBDV）VP 2 DNA疫苗被认为是一种安全的IBD预防疫苗，但其本身免疫原性较差，不能有效保护鸡群免受感染。我们和其他人证明鸡IL-2（chIL-2）和chIL-7具有增强VP 2 DNA疫苗的免疫原性的能力。然而，chIL-2和chIL-7是否可以相互增强VP 2 DNA疫苗的免疫原性，从而提高后者的保护效力仍然是未知的。通过使用chIL-2/chIL-7双顺反子基因载体与VP 2 DNA疫苗一起共免疫鸡，我们现在显示chIL-2和chIL-7显著增加IBDV VP 2特异性抗体滴度、T细胞增殖和IFN-γ产生，导致相对于单独的chIL-2或chIL-7基因载体的疫苗诱导的保护效力的最终增强。以上结果表明，chIL-2和chIL-7能够相互增强VP 2 DNA疫苗的免疫效果，为进一步优化IBDV VP 2 DNA疫苗的免疫效果奠定了基础。
Chicken infectious bursal disease (IBD) is still incompletely controlled worldwide. Although IBD virus (IBDV) VP2 DNA vaccine was considered a safe vaccine for IBD prevention, the immunogenicity by itself remains poor, resulting in the failure of effectively protecting chickens from infection. We and others demonstrated that chicken IL-2 (chIL-2) and chIL-7 have the capacity to enhance the immunogenicity of the VP2 DNA vaccine. However, whether chIL-2 and chIL-7 can mutually enhance the immunogenicity of VP2 DNA vaccine and thereby augment the latter's protection efficacy remains unknown. By using chIL-2/chIL-7 bicistronic gene vector to co-immunize the chickens together with the VP2 DNA vaccine, we now show that chIL-2 and chIL-7 significantly increased IBDV VP2-specific antibody titers, T cell proliferation, and IFN- production, resulting in the ultimate enhancement of vaccine-induced protection efficacy relative to that of chIL-2 or chIL-7 gene vectors alone. These results suggest that chIL-2 and chIL-7 can mutually enhance VP2 DNA vaccine's efficacy, thereby establishing a concrete foundation for future optimization of IBDV VP2 DNA vaccine to prevent/treat chicken IBD.