TCF21 genetic polymorphisms and breast cancer risk in Chinese women.
TCF21 genetic polymorphisms and breast cancer risk in Chinese women.
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TCF21基因多态性与中国女性乳腺癌风险
DOI：
10.18632/oncotarget.9825
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发表时间：
2016-08-23
期刊：
影响因子：
--
通讯作者：
Zhang J
中科院分区：
文献类型：
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作者：
Gao X;Yang J;Wang M;Zhang J
转录因子21（TCF 21）作为肿瘤抑制因子发挥作用，在几种类型的癌症中失活。本研究旨在探讨TCF 21基因多态性（rs2327429 T>C、rs2327430 T>C、rs2327433 A>G、rs 12190287 C>G、rs7766238 G>A、rs 4896011 T>A）与中国女性乳腺癌发病风险的关系。Logistic回归分析显示TCF 21 rs 12190287多态性与乳腺癌发病风险降低显著相关。基于病理类型的分层分析表明，TCF 21 rs 12190287多态性仅与浸润性导管癌的风险降低相关。实时荧光定量PCR分析结果显示，GG基因型乳腺癌组织中TCF 21 mRNA的表达水平高于rs 12190287 CC基因型乳腺癌组织。荧光素酶活性检测结果表明，rs 12190287 G等位基因降低了hsa-miR-224与TCF 21 3′ UTR的结合亲和力。这些结果表明，TCF 21 rs 12190287多态性可以调节TCF 21的表达，并可能作为乳腺癌遗传易感性的一个潜在的标记。
Transcription factor 21 (TCF21) functions as a tumor suppressor and is inactivated in several types of cancer. The purpose of this study is to investigate whether TCF21 genetic polymorphisms(rs2327429 T>C, rs2327430 T>C, rs2327433 A>G, rs12190287 C>G, rs7766238 G>A, rs4896011 T>A) are associated with the risk of breast cancer in Chinese women. Logistic regression analyses showed that TCF21 rs12190287 polymorphism was significantly associated with the reduced risk of breast cancer. Stratified analyses based on pathological type indicated that TCF21 rs12190287 polymorphism was only associated with the reduced risk of infiltrative ductal carcinoma. Real-time quantitative PCR analyses revealed that compared with those carrying rs12190287 CC genotype, subjects with GG genotype had higher expression levels of TCF21 mRNA in normal breast tissues. Furthermore, luciferase activity assay showed that the rs12190287 G allele weakened the binding affinity of hsa-miR-224 to TCF21 3′ UTR. These findings suggest that TCF21 rs12190287 polymorphism can regulate TCF21 expression and may serve as a potential marker for genetic susceptibility to breast cancer.