Genetic dissection of amino acid content in rice grain
Genetic dissection of amino acid content in rice grain
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DOI：
10.1002/jsfa.3731
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发表时间：
2009-11-01
影响因子：
4.1
通讯作者：
Hu, Zhongli
中科院分区：
文献类型：
--
作者：
Lu, Kaiyang;Li, Lanzhi;Hu, Zhongli
背景：稻米蛋白质含量和氨基酸组成是稻米营养品质的重要组成部分。然而，对水稻和其他作物（大豆、玉米）蛋白质和氨基酸含量的QTL定位研究报道较少。本研究以珍汕97和明恢63为亲本，利用重组自交系群体，构建了以单个氨基酸含量、必需氨基酸总量和总氨基酸总量为特征的主效应和上位效应QTL。结果：利用221个分子标记构建的连锁图，共检测到12个QTL，分别位于第1、4、6、7和11染色体上。位于第1染色体上的QTL簇（两侧为C904、R2632和C39）与8种氨基酸含量相关。单个QTL对表型变异的解释率为3.4 ~ 48.8%。81双基因相互作用的解决，涉及143个位点分布在所有12条染色体上。主效QTL所解释的变异量低于上位性QTL所解释的变异量。结论：检测到的AAC主效QTL大多位于基因组内。因此，如果育种者只对改变一种氨基酸的浓度感兴趣，这可能很难实现。同时，氨基酸含量的上位性也普遍存在。本研究中报告的信息预计将是有用的，未来的育种计划，旨在改善水稻氨基酸组成的人类营养的发展。(C)2009年化学工业协会
BACKGROUND: Protein content and amino acid composition in rice are the most important components of rice nutrient quality. However, there have been few reports on the mapping of quantitative trait loci (QTLs) for the contents of protein and amino acids in rice grain and other crops (soybean, corn). In this study a population of 241 recombinant inbred lines (RILs) from a cross between Zhenshan 97 and Minghui 63 (the parents of the most widely grown hybrid rice in China) was constructed to detect the main effect and epistatic effect QTLs for amino acid content (AAC) as characterised by individual AACs, total essential AAC and total AAC.RESULTS: Using a linkage map covering a total of 1796 centimorgan (cM) based on 221 molecular marker loci, a total of 12 QTLs were identified for ten traits mapped on chromosomes 1, 4, 6, 7 and 11. The QTL cluster (flanked by C904, R2632 and C39) on chromosome 1 was associated with the content of eight amino acids. The phenotypic variation explained by individual QTLs ranged from 3.4 to 48.8%. Eighty-one digenic interactions were resolved that involved 143 loci distributed on all 12 chromosomes. The amount of variation explained by main effect QTLs was lower than that explained by QTLs involved in epistatic interactions.CONCLUSION: The findings showed that most main effect QTLs for AACs detected tended to be co-localised within the genome. Thus, if a breeder were interested in changing the concentration of only one amino acid, this might be difficult to achieve. Meanwhile, the prevalent epistasis for the loci involved appeared to hold true for the content of amino acids. The information reported in the present study is expected to be useful for future breeding programmes targeting the development of improved rice amino acid composition for human nutrition. (C) 2009 Society of Chemical Industry