Multipotent retinal progenitors express developmental markers, differentiate into retinal neurons, and preserve light-mediated behavior
Multipotent retinal progenitors express developmental markers, differentiate into retinal neurons, and preserve light-mediated behavior
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DOI：
10.1167/iovs.04-0511
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发表时间：
2004-11-01
影响因子：
4.4
通讯作者：
Young, MJ
中科院分区：
文献类型：
--
作者：
Klassen, HJ;Ng, TF;Young, MJ
目的。从神经视网膜分离的祖细胞用于视网膜变性小鼠的移植研究。分离和鉴定出生后第1天绿色荧光蛋白转基因小鼠的视网膜祖细胞。这些细胞在培养过程中可以大量扩增，并表达神经前体细胞和/或视网膜发育的标志物。在它们被移植到成熟小鼠退化的视网膜后，视网膜前体细胞的一部分发育成成熟的神经元，包括可能表达恢复素、视紫红质或视锥视蛋白的光感受器。在Rho(-/-)宿主中，外核层(ONL)中的细胞被挽救，供体细胞广泛整合到视网膜内，与对照动物相比，受体小鼠的光介导行为有所改善。这些发现对视网膜变性的治疗有意义，其中神经元替代和光感受器挽救是主要的治疗目标。
Purpose. To use progenitor cells isolated from the neural retina for transplantation studies in mice with retinal degeneration.Methods. Retinal progenitor cells from postnatal day 1 green fluorescent protein-transgenic mice were isolated and characterized. These cells can be expanded greatly in culture and express markers characteristic of neural progenitor cells and/or retinal development.Results. After they were grafted to the degenerating retina of mature mice, a subset of the retinal progenitor cells developed into mature neurons, including presumptive photoreceptors expressing recoverin, rhodopsin, or cone opsin. In rho(-/-) hosts, there was rescue of cells in the outer nuclear layer (ONL), along with widespread integration of donor cells into the inner retina, and recipient mice showed improved light-mediated behavior compared with control animals.Conclusions. These findings have implications for the treatment of retinal degeneration, in which neuronal replacement and photoreceptor rescue are major therapeutic goals.