Heart-cutting two-dimensional liquid chromatography methods for quantification of 2-acetyl-4-(1,2,3,4-tetrahydroxybutyl)imidazole in Class III caramel colours.
Heart-cutting two-dimensional liquid chromatography methods for quantification of 2-acetyl-4-(1,2,3,4-tetrahydroxybutyl)imidazole in Class III caramel colours.
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DOI：
10.1016/j.chroma.2008.04.081
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发表时间：
2008-07
期刊：
影响因子：
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通讯作者：
Cédric Moretton;G. Crétier;H. Nigay;J. Rocca
中科院分区：
文献类型：
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作者：
Cédric Moretton;G. Crétier;H. Nigay;J. Rocca
建立了测定ⅲ类焦糖色素中2-乙酰基-4-（1,2,3,4-四羟基丁基）咪唑（THI）的二维液相色谱/紫外检测方法。在第一个维度上，用C18硅胶填料的反相色谱法或用强阳离子交换剂（SCX）固定相的离子交换色谱法对水稀释后的焦糖样品进行分馏。在每种情况下，THI洗脱结构域在一个循环中被回收，并转移到填充有多孔石墨碳（PGC）的第二柱中，用于THI的选择性分离。在接受限为20%、β-期望容差区间为90%的准确度曲线上，分别对C18-PGC和SCX-PGC两种方法在5-50和10-50ppm之间的THI/焦糖比例进行了验证。最后，比较了两种方法对大量焦糖样品的分析效果。
Heart-cutting two-dimensional liquid chromatography/UV detection methods were developed to determine 2-acetyl-4-(1,2,3,4-tetrahydroxybutyl)imidazole (noted as THI) in Class III caramel colours. In the first dimension, the caramel sample diluted in water was fractionated either by reversed-phase chromatography on C18 silica packing or by ion-exchange chromatography using strong cationic exchanger (SCX) stationary phase. In each case, THI elution domain was recovered in a loop and transferred to a second column packed with porous graphitic carbon (PGC) for the selective separation of THI. From the accuracy profiles with acceptance limit fixed at 20% and β-expectation tolerance interval fixed at 90%, the two methods, C18–PGC and SCX–PGC, were validated for THI/caramel ratios included between 5–50 and 10–50ppm, respectively. Finally, the two methods were compared for the analysis of numerous caramel samples.