THE INTEGRAL MEMBRANE-PROTEIN FROM A VIRULENT ISOLATE OF TRANSMISSIBLE GASTROENTERITIS VIRUS - MOLECULAR CHARACTERIZATION, SEQUENCE AND EXPRESSION IN ESCHERICHIA-COLI
THE INTEGRAL MEMBRANE-PROTEIN FROM A VIRULENT ISOLATE OF TRANSMISSIBLE GASTROENTERITIS VIRUS - MOLECULAR CHARACTERIZATION, SEQUENCE AND EXPRESSION IN ESCHERICHIA-COLI
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DOI：
10.1111/j.1365-2958.1988.tb00056.x
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发表时间：
1988-07-01
影响因子：
3.6
通讯作者：
GARWES, DJ
中科院分区：
文献类型：
--
作者：
BRITTON, P;CARMENES, RS;GARWES, DJ
从猪传染性胃肠炎病毒（TGEV）强毒株的亚基因组mRNA用于产生cDNA克隆。对一个新克隆和一个已报道的克隆的部分片段进行了测序，并用于构建病毒膜蛋白基因。该基因编码一个开放阅读框（ORF），编码262个氨基酸，相对分子质量（Mr）为29459。通过在E.包含编码Mr 29459多肽的大部分ORF的嵌合蛋白和β-半乳糖苷酶。嵌合蛋白与针对病毒整合膜蛋白的特异性单克隆抗体反应，并且针对嵌合蛋白产生的抗体免疫沉淀病毒蛋白。与无毒分离物的序列比较表明可能在致病性中重要的氨基酸残基。
Subgenomic mRNA from a virulent isolate of porcine transmissible gastroenteritis virus (TGEV) was used to produce cDNA clones. Part of a new clone and a previously reported clone were sequenced and used to constuct the viral gene for integral membrane protein. A single open reading frame (ORF) encoding a polypeptide of 262 amino acids, relative molecular mass (Mr) 29459, was identified. The positive identification of the polypeptide as the integral membrane protein was demonstrated by the production in E. coli of a chimaeric protein comprising most of the ORF encoding the Mr 29459 polypeptide and .beta.-galactosidase. The chimaeric protein reacted with a specific monoclonal antibody to viral integral membrane protein and antibodies raised against the chimaeric protein immune precipitated the viral protein. Comparison with the sequence of an avirulent isolate indicates amino acid residues that may be important in pathogenicity.