Laser-Doppler evaluation of rat brain microcirculation: Comparison with the [C-14]-iodoantipyrine method suggests discordance during cerebral blood flow increases
Laser-Doppler evaluation of rat brain microcirculation: Comparison with the [C-14]-iodoantipyrine method suggests discordance during cerebral blood flow increases
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DOI：
10.1097/00004647-199601000-00018
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发表时间：
1996-01-01
影响因子：
6.3
通讯作者：
Lauritzen, M
中科院分区：
文献类型：
--
作者：
Fabricius, M;Lauritzen, M
激光多普勒血流仪（LDF）是目前广泛应用的脑血流动态检测技术。我们研究了LDF测量的CBF变化是否与[C-14]碘安替比林（IAP）技术测量的CBF在大多数生理实验相关的范围内（-30-+130%）相关。在氟烷麻醉的大鼠中，通过两个激光多普勒探头记录LDF。绝对CBF测定顶叶皮质的组织样本后，[C-14]碘安替比林静脉注射。一个半球的CBF减少了皮质扩散性抑制（CSD），这显着降低了同侧皮质CBF的反应血管活性刺激长达30分钟，而对侧皮质的CBF调节保持完整。在正常通气、高碳酸血症和缺氧条件下测量CBF。LDF技术测量的CBF的相对变化与之前的基线LDF值无关。CBFIAP绝对值与同时记录的任意LDF值相关性较差（r = 0.44）。相反，CBFIAP值的比率与两个半球之间的相对LDF变化的比率相关（p < 0.001）。在降低CBF时，两种方法之间无显著差异。然而，在CBF增加时，LDF大于CBFIAP，相关斜率为1.45（p < 0.005）。
Laser-Doppler flowmetry (LDF) is very popular for measurements of dynamic changes of cerebral blood flow (CBF). We studied whether changes of CBF measured by LDF correlate with CBF measured by the [C-14]iodoantipyrine (IAP) technique in the range relevant for most physiological experiments (- 30-+ 130%). LDF was recorded biparietally by two laser-Doppler probes in halothane-anesthetized rats. Absolute CBF was measured in tissue samples of both parietal cortices after [C-14]iodoantipyrine was given i.v. CBF of one hemisphere was reduced by an episode of cortical spreading depression (CSD), which markedly reduces the responsiveness of the ipsilateral cortical CBF to vasoactive stimuli for up to 30 min, while CBF regulation of the contralateral cortex remains intact. CBF was measured under normoventilated, hypercapnic, and hypoxic conditions. The relative changes of CBF measured by the LDF technique were independent of the preceding baseline LDF value. Absolute CBFIAP values correlated poorly to the simultaneously recorded arbitrary LDF values (r = 0.44). In contrast, the ratio of CBFIAP values correlated with the ratio of the relative LDF changes between the two hemispheres (p < 0.001). At reduced CBF, no significant difference was found between methods. At increased CBF, however, LDF was greater than CBFIAP, as indicated by a slope of correlation of 1.45 (p < 0.005).