THE SIGNIFICANCE OF CARBONIC-ANHYDRASE EXPRESSION IN HUMAN COLORECTAL-CANCER
THE SIGNIFICANCE OF CARBONIC-ANHYDRASE EXPRESSION IN HUMAN COLORECTAL-CANCER
复制标题
DOI：
10.1016/0016-5085(93)90900-w
复制
发表时间：
1993-09-01
期刊：
影响因子：
29.4
通讯作者：
CHEN, LB
中科院分区：
文献类型：
--
作者：
MORI, M;STANIUNAS, RJ;CHEN, LB
背景：碳酸酐酶同工酶I和II存在于正常结直肠粘膜中。本研究旨在确定碳酸酐酶是否存在于结直肠癌及其潜在的临床意义。方法：采用北方印迹法和Western印迹法分别检测新鲜结直肠腺癌标本中碳酸酐酶同工酶I的mRNA和蛋白表达。结果：30例正常结肠组织均呈强的RNA杂交信号。39对结直肠癌和9个正常肝脏样本中只有4个检测到碳酸酐酶mRNA水平。同工酶I蛋白表达也显示了类似的结果。在另一组患者中，免疫组化研究显示96例结直肠肿瘤中有16例有阳性染色细胞。阳性肿瘤均为高、中分化癌（P< 0.05）。当回顾性分析时，免疫反应阳性的病例比阴性的病例更可能是在一组有良好的结果（P< 0.01）和缺乏血管浸润（P< 0.01）。结论：大多数大肠癌不表达碳酸酐酶同工酶I。任何同工酶I阳性免疫反应癌细胞的存在可能与结直肠癌更有利的结果。
Background:Carbonic anhydrase isoenzymes I and II are present in normal colorectal mucosa. This study aimed to determine if carbonic anhydrase is present in colorectal cancer and what is its potential clinical significance.Methods:The messenger RNA (mRNA) and protein expression of carbonic anhydrase isoenzyme I were analyzed in fresh specimens of colorectal adenocarcinoma by Northern and Western blots, respectively. The immunohistochemical expression was subsequently studied in a larger number of formalin-fixed surgical specimens.Results:All of 30 normal colon samples had a strong RNA hybridization signal. Only 4 of 39 paired colorectal cancer and none of 9 normal liver samples had detectable levels of carbonic anhydrase mRNA. Isoenzyme I protein expression showed similar results. In a separate group of patients, immunohistochemical studies showed that 16 of 96 colorectal tumors had positive staining cells. All positive tumors were well or moderately differentiated carcinomas (P< 0.05). When analyzed retrospectively, immunoreactive cases were more likely to be in a group with a good outcome (P< 0.01) and to lack vascular invasion (P< 0.01) than negative cases.Conclusions:The majority of colorectal cancers do not express carbonic anhydrase isoenzyme I. The presence of any isoenzyme I-positive immunoreactive cancer cells may be associated with a more favorable outcome in colorectal cancer.