Construction and characterization of an infectious vaccinia virus recombinant that expresses the influenza hemagglutinin gene and induces resistance to influenza virus infection in hamsters.
Construction and characterization of an infectious vaccinia virus recombinant that expresses the influenza hemagglutinin gene and induces resistance to influenza virus infection in hamsters.
复制标题
表达流感血凝素基因并诱导仓鼠对流感病毒感染产生抵抗力的感染性痘苗病毒重组体的构建和表征。
DOI：
--
复制
发表时间：
1983
影响因子：
11.1
通讯作者：
B. Moss
中科院分区：
文献类型：
--
作者：
G. Smith;B. Murphy;B. Moss
将来源于流感病毒A/JAP/305/57(H_2N_2)的流感病毒血凝素基因的DNA拷贝插入痘苗病毒早期启动子控制的痘苗病毒基因组中。用纯化的重组病毒感染组织培养细胞合成流感血凝素，经糖基化后转运到细胞表面，用胰酶将其切割成HA1和HA2亚基。皮内接种重组病毒的兔子和仓鼠产生循环抗体，抑制流感病毒的血凝。此外，接种疫苗的仓鼠获得的抗体水平与首次感染流感病毒时相似，并对A/JAP/305/57流感病毒的呼吸道感染具有保护作用。
A DNA copy of the influenza virus hemagglutinin gene, derived from influenza virus A/Jap/305/57 (H2N2) was inserted into the genome of vaccinia virus under the control of an early vaccinia virus promoter. Tissue culture cells infected with the purified recombinant virus synthesized influenza hemagglutinin, which was glycosylated and transported to the cell surface where it could be cleaved with trypsin into HA1 and HA2 subunits. Rabbits and hamsters inoculated intradermally with recombinant virus produced circulating antibodies that inhibited hemagglutination by influenza virus. Furthermore, vaccinated hamsters achieved levels of antibody similar to those obtained upon primary infection with influenza virus and were protected against respiratory infection with the A/Jap/305/57 influenza virus.