Biological and Phylogenetic Characteristics of Yellow Fever Virus Lineages from West Africa
Biological and Phylogenetic Characteristics of Yellow Fever Virus Lineages from West Africa
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DOI：
10.1128/jvi.01116-12
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发表时间：
2013-03-01
影响因子：
5.4
通讯作者：
Sall, Amadou A.
中科院分区：
文献类型：
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作者：
Stock, Nina K.;Laraway, Hewad;Sall, Amadou A.
黄热病病毒（YFV）是第一个被证实的人类致病病毒，尽管在1927年被分离出来，但仍然是一个主要的公共卫生问题，特别是在西非，它每年都会引起疫情。然而，很少有人知道它的遗传多样性和进化动力学，主要是由于数量有限的野生病毒分离株的基因组序列。在这项研究中，我们分析了24个全长基因组的系统发育关系，从YFV株分离于1973年和2005年之间的森林背景下，西非，包括14个分离株，以前没有被测序。通过这一点，我们证实了一个基因型内的遗传变异的各种YF谱系在西非流通的鉴定。对这些谱系的生物学特性的进一步分析揭示了人类肝脏和昆虫细胞中的差异生长行为，这与分离的来源相关，并表明宿主适应。对于一个谱系，在垂直传播的背景下反复分离，观察到病毒基因组的生长行为和独特突变的特定特征，值得进一步研究，以深入了解自然界中YFV出现和维持的机制。
The yellow fever virus (YFV), the first proven human-pathogenic virus, although isolated in 1927, is still a major public health problem, especially in West Africa where it causes outbreaks every year. Nevertheless, little is known about its genetic diversity and evolutionary dynamics, mainly due to a limited number of genomic sequences from wild virus isolates. In this study, we analyzed the phylogenetic relationships of 24 full-length genomes from YFV strains isolated between 1973 and 2005 in a sylvatic context of West Africa, including 14 isolates that had previously not been sequenced. By this, we confirmed genetic variability within one genotype by the identification of various YF lineages circulating in West Africa. Further analyses of the biological properties of these lineages revealed differential growth behavior in human liver and insect cells, correlating with the source of isolation and suggesting host adaptation. For one lineage, repeatedly isolated in a context of vertical transmission, specific characteristics in the growth behavior and unique mutations of the viral genome were observed and deserve further investigation to gain insight into mechanisms involved in YFV emergence and maintenance in nature.